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Technologies

Research

Our research projects rely on several (bio-)analytical, synthetic and biochemical techniques and methods. They are continuously progressed by our own research and for our collaborators:


Mass Spectrometry

  • MALDI-TOF/TOF-MS, ESI-QqTOF-MS, ESI-LTQ-Orbitrap-MS
  • LC/LC-ESI/MALDI-MS-coupling; CE-ESI-MS-coupling
  • de-novo-Sequencing of peptides
  • posttranslational modified peptides and proteins



  • Peptide Synthesis

  • Fmoc-strategy
  • automated parallel and microwave assisted solid phase synthesis
  • modified peptides (side-chains, back-bone, amino-acid synthesis)
  • peptide-ligation techniques
  • imunogens + epitope mapping


  •    
    Proteomics

  • 1- and 2-dimensional gel electrophoresis techniques
  • gel staining (Coomassie, silver, fluorescence)
  • (immuno-) blotting-techniques (highly sensitive detection techniques; different software-packages for data processing)
  • automated spot-cutting
  • automated in-gel digestion
  • mass spectrometry



  • ELISAs

  • direct and sandwich-techniques
  • Immuno-PCR


  •    
    Separation Techniques

  • HPLC (RPC, HILIC, HIC, IEC, SEC); starting from nano- and capillary-LC to analytical and preparative techniques; multi-dimensional techniques and MS-coupling
  • gel electrophoresis (SDS-PAGE, native electrophoresis, IEF, 2-DE)
  • capillary electrophoresis

  • Cell Culture Techniques
    Fluorescence Microscopy
    Fluorescence Polarization
    (Anisotropic Polarization)

       
     
    home- privacy Bioanalytik, bioanaly@rz.uni-leipzig.de, 01.06.2018