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Scientific Course "Cell Methods"

The 3-day-course “Cell Methods” provides special techniques of cellular functional analysis, e.g. advantages and disadvantages of various cell culture systems, their fields of application and limitations.

Schedule

14 September

9.00 - 10.00 amLecture: Blood cells and their separation
Dr. Sandra Franz
 
10.00 - 1.00 pm 
Practical training: Cell separation from human blood
Dr. Sandra Franz/PD Dr. Anja Saalbach

2.00 - 4.00 pm

 

Practical training: Analysis of human blood cells
Dr. Sandra Franz/PD Dr. Anja Saalbach
 4.30 - 5.30 pm Lecture: Interaction of immune system with biomaterials
Prof. Dr. Axel Roers

15 September

9.00 - 10.00 am
Lecture: Cell analysis by flow cytometry
PD Dr. Anja Saalbach
10.15 - 11.45 am
Practical training: Cell analysis by flow cytometry
Dr. Sandra Franz/PD Dr. Anja Saalbach
12.00 - 1.00 pm
Lecture: Gene expression analysis from cells to Ct
PD Dr. Ulf Anderegg
2.00 - 4.00 pm
Practical training: RNA purification and cDNA synthesis
PD Dr. Ulf Anderegg
4.30 - 5.30 pm
Lecture: Apoptosis and necrosis of leukocytes during inflammation
Prof. Dr. Jürgen Arnhold

16 September

9.00 - 11.00 am
Practical training: Realtime qPCR
PD Dr. Ulf Anderegg
11.30 - 1.00 pm
Lecture: Cell census - methods for normalization of in vitro data
Dr. Ute Hempel
2.00 - 3.30 pm
Practical training: Realtime qPCR, data analysis
PD Dr. Ulf Anderegg
3.30 - 4.30 pm
Discussion/open questions
Dr. Sandra Franz/PD Dr. Anja Saalbach/PD Dr. Ulf Anderegg

7 October

9.30 - 11.00 am

Final written examination

 

Venue:

Max-Bürger-Forschungszentrum, Johannisallee 30, Seminar room B 115

Setup:
Maximum number of participants: 10
Credit points: 2 (graded)

Please register until 30 August by sending an e-mail to Anja Pohl.