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Cell Methods

Das Modul vermittelt spezielle Technikern der zellulären Funktionsanalyse, die im Transregio verankert sind. Es werden die Vor- und Nachteile der verschiedenen Zellkultursysteme, deren Einsatzmöglichkeiten und Limitierungen vorgestellt. "Cell Methods" findet vom 03. bis 05. September 2014 in Dresden statt.

Ablaufplan

03 Sept. 2014 [1]
10:00 – 12:00
Lecture:
Destructing Bone - Osteoclasts
(Martina Rauner)
12:00 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoclasts I (
Martina Rauner)
[1] Generation of Primary Murin Osteoclasts   
[2] TRAP Staining

[3] TRAP Activity Measurement
   
16:00 – 18:00
Lab Course:
Characterization of Osteoclasts II (
Martina Rauner)
[1] Generation of Primary Murin Osteoclasts   
[2] TRAP Staining

[3] TRAP Activity Measurement
   

04 Sept. 2014 [2 + 3]
08:00 – 10:00
Lecture (Seminarraum 1):
Fixed or Mobile? - Measurement of Cell Force and Migration

(Tilo Pompe)

10:00 – 12:00
Lecture (Seminarraum 1):
Building Bone - Osteoblasts
(Ute Hempel)
12:00 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoblasts I (Ute Hempel)
[4] Staining for TNAP and CaP
[5] TNAP Activity and Ca Quantitation
[6] Immunoflourescence Staining and Microscopy
16:00 – 18:00
Lab Course:
Characterization of Osteoblasts II (Ute Hempel)
[4] Staining for TNAP and CaP
[5] TNAP Activity and Ca Quantitation
[6] Immunoflourescence Staining and Microscopy

05 Sept. 2014 [2 + 3]
08:00 – 10:00

Lab Course:
Documentation

(Ute Hempel)

10:00 – 12:00
Lecture (Seminarraum 2):
In the inner circle - Real Time Quantitative Polymerase Chain Reaction (qPCR)
(Ulf Anderegg)

12:00 – 14:00
Lecture (Seminarraum 2):
Beneficial or not? - Reactive oxygen species in immune cells
(Jürgen Arnhold)
14:00 – 15:00
Lunch Break
15:00 – 16:00
Final Remarks and Discussion (Seminarraum 2)
(Martina Rauner and Ute Hempel)

19 Sept. 2014
 

Written Performance Control

Please prepare a detailed protocol according the following guidelines taking into account the following questions as well as the scientific lectures and Lab Courses (max. 10 pages)

  • Topic
  • Aim/Introduction
  • Materials and Methods (background)
  • Results
  • Short discussion of results

Please send the LAB-REPORT until September 19 to albrecht@uni-leipzig.de. 

Download:
[1] Template LAB-REPORT (Word, 70KB)
[2] Script (PDF, 12MB)
[3] Pictures (PDF, 2MB)


 

Ort:
[1] LECTURE & LAB COURSE - TU Dresden, Universitätsklinikum, Fetschestraße 74, Haus 19 (5. OG), 01307 Dresden
[2] LECTURES - TU Dresden, Medizinisch-Theoretisches Zentrum, Institut für Physiologische Chemie,  Seminarraum 1 / 2,  Fiedlerstraße 42, 01307 Dresden
[3] LAB COURSE  - TU Dresden, Medizinische Fakultät, Medizinisch-Theoretisches Zentrum, Institut für Physiologische Chemie, Fiedlerstraße 42, 01307 Dresden


Organisatorisches:
Maximale Teilnehmerzahl: 10    
Credit points: 2 (benotet)

Anmeldung:
Die Anmeldung erfolgt im Zeitraum vom 15. - 30. August 2014 per e-Mail an Anett Albrecht.