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Cell Methods

The 3-days course "Cell Methods" provides special techniques of cellular functional analysis, e.g. advantages and disadvantages of various cell culture systems, their fields of application and limitations. The "Cell Methods" Course takes place in Dresden from 3 to 5 September 2014.

Schedule

03 Sept. 2014 [1]
10:00 – 12:00
Lecture:
Destructing Bone - Osteoclasts
(Martina Rauner)
12:00 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoclasts I (
Martina Rauner)
[1] Generation of Primary Murin Osteoclasts   
[2] TRAP Staining

[3] TRAP Activity Measurement
   
16:00 – 18:00
Lab Course:
Characterization of Osteoclasts II (
Martina Rauner)
[1] Generation of Primary Murin Osteoclasts   
[2] TRAP Staining

[3] TRAP Activity Measurement
   

04 Sept. 2014 [2 + 3]
08:00 – 10:00
Lecture:
Fixed or Mobile? - Measurement of Cell Force and Migration

(Tilo Pompe)

10:00 – 12:00
Lecture:
Building Bone - Osteoblasts
(Ute Hempel)
12:00 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoblasts I (Ute Hempel)
[4] Staining for TNAP and CaP
[5] TNAP Activity and Ca Quantitation
[6] Immunoflourescence Staining and Microscopy
16:00 – 18:00
Lab Course:
Characterization of Osteoblasts II (Ute Hempel)
[4] Staining for TNAP and CaP
[5] TNAP Activity and Ca Quantitation
[6] Immunoflourescence Staining and Microscopy

05 Sept. 2014 [2 + 3]
08:00 – 10:00

Lab Course:
Documentation

(Ute Hempel)

10:00 – 12:00
Lecture:
In the inner circle - Real Time Quantitative Polymerase Chain Reaction (qPCR)
(Ulf Anderegg)

12:00 – 14:00
Lecture:
Beneficial or not? - Reactive oxygen species in immune cells
(Jürgen Arnhold)
14:00 – 15:00
Lunch Break
15:00 – 16:00
Final Remarks and Discussion
(Martina Rauner and Ute Hempel)

19 Sept. 2014
 

Written Performance Control

Please prepare a detailed protocol according the following guidelines taking into account the following questions as well as the scientific lectures (max. 10 pages)

  • Topic
  • Aim/Introduction
  • Materials and Methods (background)
  • Results
  • Short discussion of results

 

Venues:
[1] LECTURE & LAB COURSE  - TU Dresden, Medizinische Fakultät, Universitätsklinikum, Fetschstraße 74, Haus 19, 5 OG, 01307 Dresden

[2] LECTURES - MITZ - Medizinisches Interprofessionelles Trainingszentrum, (evt. Kleiner Hörsaal), Blasewitz Straße, 01307 Dresden
[3] LAB COURSE  - TU Dresden, Medizinische Fakultät, Medizinisch-Theoretisches Zentrum, Institut für Physiologische Chemie, Fiedlerstraße 42, 01307 Dresden


Setup:
Maximum number of participants: 10
Credit points: 2 (graded)

Registration:
Please register until 30 August by sending an e-mail to Anett Albrecht.