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Cell Methods (advanced)

Das Modul vermittelt spezielle Technikern der zellulären Funktionsanalyse, die im Transregio verankert sind. Es werden die Vor- und Nachteile der verschiedenen Zellkultursysteme, deren Einsatzmöglichkeiten und Limitierungen vorgestellt. "Cell Methods" findet vom 23. bis 25. November 2016 in Dresden statt.

Ablaufplan

23. Nov. 2016 [1]
10:00 – 12:00
Lecture:
Destructing Bone - Osteoclasts
(Martina Rauner)
12:00 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoclasts (
Martina Rauner)
[1] Generation of Primary Murin Osteoclasts   
[2] TRAP Staining

[3] TRAP Activity Measurement
   

24. Nov. 2016 [2]
09:00 – 11:00
Lecture:
Fixed or Mobile? - Measurement of Cell Force and Migration

(Tilo Pompe)

11:00 – 12:30
Lecture:
Building Bone - Osteoblasts
(Ute Hempel)
12:30 – 14:00
Lunch Break
14:00 – 16:00
Lab Course:
Characterization of Osteoblasts
(Ute Hempel)
[4] Staining for TNAP and CaP
[5] TNAP Activity and Ca Quantitation
[6] Immunoflourescence Staining and Microscopy

It is possible to use and characterize own materials. 

25. Nov. 2016 [3]
09:00 – 10:00

Lab Course:
Documentation

(Ute Hempel)

10:00 – 12:00
Lecture:
In the inner circle - Real Time Quantitative Polymerase Chain Reaction (qPCR)
(Ulf Anderegg)

12:00 – 14:00
Lecture (Seminarraum 2):
Beneficial or not? - Reactive oxygen species in immune cells
(Jürgen Arnhold)
14:00 – 15:00
Lunch Break
15:00 – 16:00
Final Remarks and Discussion
(Martina Rauner and Ute Hempel)

15. Dec. 2016
 

Written Performance Control

Please prepare a detailed protocol according the following guidelines taking into account the following questions as well as the scientific lectures and Lab Courses (max. 10 pages)

  • Topic
  • Aim/Introduction
  • Materials and Methods (background)
  • Results
  • Short discussion of results

Please send the LAB-REPORT until December 15 to albrecht@uni-leipzig.de.


 

Venue:
TU Dresden, Medizinische Fakultät, Medizinisch-Theoretisches Zentrum, Institut für Physiologische Chemie, Fiedlerstraße 42, 01307 Dresden

[1] Seminarraum 1 im MTZ (Mittwoch)
[2] Seminarraum 5 im MTZ (Donnerstag)
[3] Seminarraum 108 im Dekanatsgebäude (Freitag) 

Number of Participants: 10    
Credit points: 2 (benotet)

Registration:
Please register by sending an E-Mail to Anett Albrecht.

Accommodation

For Students we could offer Singlerooms in shared flats (2 persons) at the International Guest House (Hochschulstr. 50, 1069 Dresden). The rooms are fully furnished, with kitchen and bathroom, including beelines and towels. The overnight fees are 30€/person (optional + 5,50€ breakfast). Please make your booking before September 30, 2016 via online-Registration. Please add the keyword „Doktoranden Leipzig“ in the field "Erläuterung".

ONLINE REGISTRATION