DAAD RISE Programm: CALL 2017

Monday, 26. September 2016 10:16 | Author:

DAAD RISE Programm The German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) announced a new call for its program “Research Internships in Science and Engineering” (RISE) for summer internship in 2017.

The program works in both ways. German and American Ph.D. students submit internship offers for undergraduates students for a duration of 8 to 12 weeks. German bachelor students can work as research interns at U.S. universities and U.S. undergraduates can work in labs of German universities. The DAAD provides stipends to help cover living expenses, the partner universities and research institutes provide housing assistance. The call is applicable for all fields of biology, chemistry, physics, earth sciences and engineering.

Please visit for further information:

DAAD RISE DAAD RISE worldwide
To promote the exchange of students from North America and Great Britain to Germany in the fields of natural science, engineering and life sciences To enable young German scientists to conduct research abroad
   
Offering Research Internship in Germany Offering Research Internship worldwide
October 1, 2016 – November 15, 2016 September 1, 2016 – October 15, 2016
   
Apply for Research Internship in Germany Apply for Research Internship worldwide
December 1, 2016 – Januar 15, 2017 November 1, 2016 – December 22, 2016
   

Students and researchers from Leipzig and Vanderbilt Universities with an interest in exchange activities with the other partner university are kindly asked to contact Ms. Anett Albrecht for more information and assistance.

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Short report by Henrike Indrischek: BINDING CAPABILITY OF VISUAL ARRESTINS TO THE COATED PIT MACHINERY at Vanderbilt University (May 05 – July 28)

Thursday, 4. August 2016 8:50 | Author:

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by Henrike Indrischek (phd student at Leipzig University): During my research stay at Vanderbilt university this summer, I worked on the biochemical characterization of the interaction of clathrin and visual arrestin in the laboratory of Seva Gurevich and the modeling of this interaction with computational methods in the laboratory of Jens Meiler.

The cytosolic arrestin proteins mediate desensitization of activated G protein-coupled receptors (GPCRs) via competitive binding to the active phosphorylated receptor. As different arrestin conformations can result in specific signaling outcomes, this protein family is an attractive target in drug therapeutics. Non-visual arrestins mediate uptake of the receptors into the cell by also binding clathrin, a component of the endocytosis machinery. Conservation of the low affinity clathrin binding site of one of the visual arrestins suggested a possible role in clathrin-mediated endocytosis. I wanted to use the reasearch stay at Vanderbilt to follow up on this hypothesis with biochemical and computational methods from structural biology.

I especially enjoyed spending time in an experimental laboratory working ‘hands-on’ again after a long time of computational work. The people in the Gurevich lab did an excellent job in welcoming me in the group and in explaining me methods and techniques, that I had not used in a long time, e.g. Western Blot. Apart from the scientific experience, I spent a really good time with amazing people, both from the Meiler and Gurevich lab! This included typical American activities like watching a baseball game, eating out at Southern style restaurants, having a BBQ, going out on Nashville’s famous broadway. Everybody has been very friendly (definitely exceeding Southern style-small talk friendliness) so that leaving Nashville also ment leaving new and old friends. – Thank you, Sandra, Sergey and Alyssa!!!

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Researchers of Vanderbilt and Leipzig University discover small-molecule modulators of the human Y4 receptor

Monday, 4. July 2016 8:49 | Author:

pubpic_071604(07/04/2016 by Anett Albrecht): A joint publication of Leipzig and Vanderbilt scientists was published in June 2016 in the prestigious journal “PLoS One”. The article continues the series of numerous publications that have emerged already from the long-term collaboration between the Leipzig and Vanderbilt University.

Abstract: The human neuropeptide Y4 receptor (Y4R) and its native ligand, pancreatic polypeptide, are critically involved in the regulation of human metabolism by signaling satiety and regulating food intake, as well as increasing energy expenditure. Thus, this receptor represents a putative target for treatment of obesity. With respect to new approaches to treat complex metabolic disorders, especially in multi-receptor systems, small molecule allosteric modulators have been in the focus of research in the last years. However, no positive allosteric modulators or agonists of the Y4R have been described so far. In this study, small molecule compounds derived from the Niclosamide scaffold were identified by high-throughput screening to increase Y4R activity. Compounds were characterized for their potency and their effects at the human Y4R and as well as their selectivity towards Y1R, Y2R and Y5R. These compounds provide a structure-activity relationship profile around this common scaffold and lay the groundwork for hit-to-lead optimization and characterization of positive allosteric modulators of the Y4R.

Publication: Sliwoski G, Schubert M, Stichel J, Weaver D, Beck-Sickinger AG, Meiler J. Discovery of Small-Molecule Modulators of the Human Y4 Receptor. PLoS One. 2016 Jun 13;11(6):e0157146. doi: 10.1371/journal.pone.0157146.

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Announcement: Lecture on “Regulation of Exocytosis by inhibitory GPCRs and Gbg subunits” at Leipzig University

Monday, 4. July 2016 7:57 | Author:

pic_0716Biochemical Colloquium – Special Topics in Biochemistry

Prof. Dr. Heidi Hamm
, Vanderbilt University Department of Pharmacology, Nashville, TN, USA

Date July 13, 2016 / 3 PM

Venue Departments of Bioscience, Pharmacy and Psychology, Institute of Biochemistry, Small Lecture Hall (1st Floor), Brüderstraße 34, 04103 Leipzig, Germany.

Backround
Prof. Hamms work is focused on understanding the molecular basis of signaling mechanisms mediated by G proteins, which are switch proteins. G proteins are normally inactive, but a receptor that has received a specific signal can activate G proteins, leading to changes in the activity of enzymes that produce second messengers such as cyclic AMP and calcium. The resulting changes in cellular activity underlie a large number of physiological processes. G protein-mediated signalling cascades are key regulators of many physiological processes, including processes of development, differentiation, and regulation of cell division.

Link
https://medschool.vanderbilt.edu/hamm-lab/

Participation
The lecture is open for students, phds, postdocs or group memebers and are cordially invited to attend.

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Announcement: Course on “IN VITRO PREPARATION OF MEMBRANE PROTEINS – FROM TARGET DNA TO NMR SPECTRA” at Vanderbilt University

Sunday, 1. May 2016 13:54 | Author:

PSchmidtGraduate Course – Special Topics in Protein Biochemistry

Dr. Peter Schmidt, Leipzig University, Faculty of Medicine, Institute for Medical Physics and Biophysics

Date
The class will meet on six days in July always 9:35-12:15. Dates will be finalized with class participants. This is a one credit course.

Venue
Departments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, 5131 MRBIII, Nashville, TN 37232-8725

List of Topics:
– Introduction into genetic engineering and recombinant expression (1h)
– Membrane proteins, GPCRs, and Y receptors (1h)
– Host systems for recombinant membrane protein expression and fermentation process (2h)
– Isolation and purification of membrane proteins (1h)
– Artificial membrane environments (1h)
– In vitro folding and reconstitution of membrane proteins (1h)
– Activity assays at different concentrations (1h)
– Labeling membrane proteins for NMR spectroscopy (1h)
– Basic introduction into NMR spectroscopy and product operators (2h)
– 1D-, 2D-, and 3D-NMR (1h)
– NMR experiments: HSQC, DQF, PDSD, a.o. (3h)

Registration
Students who wish to take the class for credit should register as soon as possible! Students, postdocs, and faculty who wish to audit the class are welcome. Please send a note to Jens Meiler (jens.meiler@vanderbilt.edu).

Grading
There will be a written exam at the end of class.

read more…

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Journal of Biological Chemistry selects Vanderbilt-Leipzig article as paper of the week

Monday, 14. March 2016 11:08 | Author:

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The joint publication of Hu, Stern, Gimenez, Wanka, Zhu, Rossi, Meister, Inoue, Beck-Sickinger, Gurevich and Wess was named as “Paper of the Week” by the Journal of Biological Chemistry’s editorial board, landing it in the top 2 percent of all papers published over the year in the journal.

The joint publication focussed on designer receptors exclusively activated by a designer drug – short DREADDs. This are CNO sensitive designer G protein-coupled receptors which are able to activate heterotrimeric G proteins and also trigger arrestin-dependent signaling. In the present study the development of a mutationally modified version of a non-biased DREADD derived from the M3 muscarinic receptor that can activate Gq/11 with high efficacy but lacks the ability to interact with arrestins. This novel Gq/11-biased DREADD represents a powerful new tool to study the physiological relevance of Gq/11-dependent signaling in distinct tissues and cell types, in the absence of arrestinmediated cellular effects.

The great advantage of DREADD technology is that it allows the activation of distinct signaling pathways in a particular cell type in vivo. As a consequence, such DREADD studies offer direct insights into the cellular basis of an observed physiological or behavioral response.

Publication: Hu, Stern, Gimenez, Wanka, Zhu, Rossi, Meister, Inoue, Beck-Sickinger, Gurevich, Wess (2016): A G Protein-Biased Designer G Protein-Coupled Receptor Useful for Studying the Physiological Relevance of Gq/11-Dependent Signaling Pathways. JBC. February 5, 2016. doi: 10.1074/jbc.M115.702282.

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Short report by Mario Schubert: HTS TO IDENTIFY AND CHARACTERIZE SMALL MOLECULE ALLOSTERIC MODULATORS OF THE HUMAN Y4 RECEPTOR at Vanderbilt University (Oct 19 – Dec 15)

Tuesday, 8. March 2016 12:45 | Author:

vuluschubert122015_group During an internship in the lab of Dave Weaver at Vanderbilt University, I got the opportunity to gain more information about the Y4R, by testing a variety of similar structurally related compounds. I was introduced in the workflow of a high throughput screening laboratory. The technical equipment in the Vanderbilt HTS center makes it possible to test thousands of different chemical compounds in a very short time. Therefore, I learned to perform Ca2+-flux based signal transduction screening assays to monitor the activation of the Y4R. Using this system, I could test new hit compounds out of the ongoing screening for activity and selectivity. Furthermore, I tested structurally similar compounds of a previous screening hit for a Y4R selective positive allosteric modulator and could identify structural regions that are important for the activity. I learned different ways to organize and evaluate the large amounts of HTS data using different kinds of software for qualitative and quantitative analysis. At all of these steps, Dave and his group members kindly introduced me in the new methods. I also was able take a look on devices under development, giving me a very interesting impression what is possible in the current state of HTS-technology.

My lab colleagues not only introduced me in the lab, but also in the American lifestyle. I really enjoyed the relaxed atmosphere in the monthly happy hours with all lab members. They took me around to the special places of Nashville. We enjoyed listening country music in different bars, watching football games, had good training session in the sports center and nice shopping trips in the big malls.

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Short Report by Axel Fischer & Gregory Sliwoski: INTEGRATION OF LIMITED EXPERIMENTAL DATA INTO ENSEMBLE PREDICTION at Leipzig University (Nov 14 – Dec 22)

Monday, 15. February 2016 12:49 | Author:

vulu_luvoelki15The objective of our visit was deepening the collaboration between Leipzig University and Vanderbilt University regarding the integration of limited experimental data into protein ensemble prediction.

During this visit Axel Fischer presented a method to integrate cross-linking data into protein structure prediction methods at the 5th Symposium on Structural Proteomics in Halle. Following subsequent discussions with Dr. Stefan Kalkhof and Tommy Hofmann, work on an algorithm to efficiently simulate cross-link conformations was continued. In a preliminary study, the algorithm significantly improves the accuracy with which protein structures can be predicted.

Additionally, Axel developed an algorithm to integrate solid-state nuclear magnetic resonance (ssNMR) data into the protein structure prediction software BCL::Fold. While the algorithm still has to thoroughly benchmarked on experimental data, incorporation of ssNMR data will provide information about the tilt angles and rotation states of transmembrane helices, which will improve the accuracy of protein structure prediction methods.

Gregory Sliwoski met with collaborators Prof. Dr. Torsten Schöneberg and Dr. Antje Brüser to discuss the experimental results of mutations suggested by computational ligand-receptor models and devised strategies to incorporate these results into further rounds of protein modeling and ligand docking. Additionally, Gregory successfully defended his thesis titled “Computer Aided Drug Discovery: Descriptor improvement and application to obesity-related therapeutics” at the Universität Leipzig.

During this stay we enjoyed Gluehwein at the Christmas Market at the Connewitzer Kreuz. We also visited the Voelkerschlacht Denkmal. Managing to climb to its top provides a beautiful view over Leipzig.

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Scientists of Vanderbilt and Leipzig University improved drug effects of Indomethacin

Thursday, 4. February 2016 8:29 | Author:

VULU12584638(Leipzig/Nashville, 02/03/2016): Scientists at Leipzig University and Vanderbilt University (USA) reduced the side effects of Indomethacin by altering the so-called boron cluster molecule of this drug. This molecule have a spherical shape – composed of boron, hydrogen and carbon atoms. The active ingredient causes an inhibition of the enzyme cyclooxygenase, which plays an important role in production of inflammations and pain. But Indomethacin also leads to severe side effects. The scientists changed the drug, so that it blocks only the pathological form of the enzyme. The other form, which controls important physiological processes in the body, would barely be affected.

The results underline the potential of boron clusters in pharmacytical application and could contribute to drugs development. They published their results in the prestigious journal “ChemMedChem”.


Publication: Neumann, Xu, Sárosi, Scholz, Crews, Ghebreselasie, Banerjee, Marnett, Hey-Hawkins (2016): nido-Dicarbaborate Induces Potent and Selective Inhibition of Cyclooxygenase-2. ChemMedChem. 11:2016, 175–178. doi: 10.1002/cmdc.201500199.


The article continues the series of numerous publications that have emerged already from the long-term collaboration between the Leipzig University and Vanderbilt University.

Press Release: Chemiker verändern Schmerzwirkstoff – Hoffnung auf weniger Nebenwirkungen durch Borcluster (Febr. 03, 2016)

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Professor Jens Meiler received Friedrich Wilhelm Bessel Research Award

Friday, 18. December 2015 9:00 | Author:

JensMeiler(by David Salisbury, Vanderbilt University): Associate Professor of Chemistry Jens Meiler has received a Friedrich Wilhelm Bessel Research Award from the Alexander von Humboldt Foundation in Bonn, Germany.

The award is given to scholars based on their “outstanding research accomplishments and exceptional promise for the future.” Individuals are nominated by German scholars and must have received their Ph.D. in the last 18 years.

In addition to a monetary prize, award winners are invited to spend a period of up to one year collaborating on a long-term research project. Meiler, who has an international reputation in the field of structural biology, will be collaborating with colleagues at Leipzig University on studies of G-protein coupled receptors, a class of sensor molecules that are often targeted by therapeutics and include the pain medication hydrocodone and lisinopril, which is used to treat high blood pressure.

Meiler has been one of the leaders of a grass-roots faculty effort to establish a collaboration with his alma mater, Leipzig University, which led the two universities to sign a memorandum of understanding encouraging academic exchanges between the two campuses.

The Humboldt Foundation grants about 20 Friedrich Wilhelm Bessel Research Awards annually. The award is valued at $49,500.

Press Release: Chemist Jens Meiler receives Humboldt Foundation award (Dec. 15, 2015)
Press Release: Hochkarätige Auszeichnung für Gastforscher an Universität Leipzig (Nov. 05, 2015)

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Short Report by Brain Bender: EXPRESSION OF Y2R VARIANTS FOR EPR STRUCTURAL STUDIES at Leipzig University (Aug 21 – Oct 14)

Tuesday, 1. December 2015 10:15 | Author:

BB04This fall I was awarded the opportunity to return to Leipzig University to work in the lab of Dr. Daniel Huster. This research exchange corresponded nicely with the 11th International NPY-PYY-PP Conference. At the conference I was able to interact with many people in the field of neuropeptide Y (NPY) and its related peptide hormones. At this conference, people from many diverse fields came together to discuss their research. I heard talks on the role of NPY in cancer, obesity, and development of new receptor agonists. This greatly expanded on my knowledge of the physiological role of NPY and its receptors in the brain and throughout the body. One really great session explored the evolutionary history of the NPY receptors and also evolutionary interconnectedness of pre-human cultures. The night of NPY evolution was capped off by a trip to the Leipzig Zoo where we got to see many of the wonderful animals living there before enjoying dinner in a safari-like setting. It was really nice to see a number of talks about the structure of these peptides and receptors.

Following the conference I was able to dive head first into the lab with the continued excitement from the conference. In the lab my goal was to express and purify NPY type 2 receptor (Y2R) for the use in electron paramagnetic resonance (EPR). EPR introduces paramagnetic spin labels via nitoxides at specific amino acid residues in the protein. Introduction of two EPR labels into a protein or protein complex allows for double electron-electron resonance (DEER) spectroscopy which measures the distance between these two labels. By positioning pairs of EPR tags throughout the protein, I can build a model of the receptor using these distance restraints.

Any research trip to a foreign country lends the opportunity to explore a different culture. I set out on my quest to continue exploring German pastries and beers which cumulated in a trip to “Oktoberfest” in Munich. Further, I visited Nurenberg to see the castle and a museum at the Documentation Center, a fascinating history lesson. Lastly, I visited one of Germany’s neighboring countries, Belgium, to visit a student who had done an exchange in the Meiler lab in Vanderbilt last year. While there I partook of Belgium waffles and chocolates and watched an incredible festival of lights fireworks display.

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APPLY FOR MAX KADE SHOLARSHIP: Unique Opportunities in Gaining Research Experience in Germany and USA

Thursday, 19. November 2015 8:47 | Author:

vulu_web

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three undergraduate students from each university to conduct a 10 week summer internship at the respective partner university in 2016 (May – September). The Max Kade Foundation Scholarship Program is open for highly motivated and excellent foreign applicants with a BSc, MSc or equivalent degrees (obtained during the past year).

Internship offer in Leipzig for Undergraduate Students at Vanderbilt University (Date: May – August 2016, Project Descriptions 2016)

  1. Genetic Incorporation of Photocrosslinking Amino Acids: Binding Pattern of β-Arrestins on a Class B GPCR
    Laboratory Principal Investigator: Dr. Irene Coin
    Graduate Mentor in host Laboratory: Lisa Seidel
  2. Paramagnetic solid-stae NMR spectroscopy on NPY/Y receptor complex
    Laboratory Principal Investigator: Prof. Dr. Daniel Huster
    Graduate Mentor in host Laboratory: Dr. Peter Schmidt
  3. Investigation of NPY5R-Ligand binding
    Laboratory Principal Investigator: Prof. Dr. Annette Beck-Sickinger
    Graduate Mentor in host Laboratory: Tristan Zellmann

Internship offer in Nashville for Master Students at Leipzig University, (Date: July – September 2016, Project Descriptions 2016)

  1. Structural Basis of Arrestin-2 signaling
    Laboratory Principal Investigator: Prof. Dr. Vsevolod Gurevich
    Post-Doctoral Mentor in host Laboratory:  Dr. Sandra Berndt
  2. Structure Refinement of α-helical Membrane Protein by Paramagnetic LAN-thanide Ion tagging
    Laboratory Principal Investigator: Prof. Dr. Jens Meiler
    Post-Doctoral Mentor in host Laboratory: Soumya Ganguly
  3. New Chemical Probes for Monitoring Ion Channels and other in Cell Events
    Laboratory Principal Investigator: Prof. Dr. Gary Sulikowski (Chemistry) and Prof. Dr. Dave Weaver (Pharmacology)
    Post-Doctoral Mentor in host Laboratory: Brendan Dutter

The applicant is required to compile a single pdf document including the form (background), a rank list of the projects available, a CV, an unofficial transcript of courses including the current GPA, a statement summarizing past research experience and the motivation to pursue research on this project.

Please submit your application materials as a single PDF until December 31 to Mrs Heather Darling (VU, h.darling@vanderbilt.edu) and Mrs. Anett Albrecht (LU, albrecht@uni-leipzig.de).

Three candidates from each university are selected by reviewing the application materials and conducting personal interviews.

More Information

Max-Kade Foundation Scholarship 2015
Short Reports 2015
Internship offer 2015 at Leipzig University
Internship offer 2015 at Vanderbilt University

Max-Kade Foundation Scholarship 2014
Short Reports 2014

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Max-Kade Scholarship 2015: Short-Reports

Monday, 9. November 2015 6:05 | Author:

MKF2015_Nashville_Leipzig

In 2015 eight students on Bachelor- or Master level got the Max-Kade Scholarship to participate in ongoing research projects between Vanderbilt University and the University of Leipzig.

Ryan Xin (Undergraduate Student at Vanderbilt University):
at Laboratory of Prof. Dr. Annette Beck-Sickinger and Stefan Ernicke

Michelle Chapmann (Undergraduate Student at Vanderbilt University):
at Laboratory of Prof. Dr. Norbert Sträter and Marco Stelter, Ulrike Obeck, Björn Kieslich

Michael Mercier (Undergraduate Student at Vanderbilt University):
at Laboratory of Prof. Dr. Daniel Huster and Dr. Ulrike Krug

Han Noo Ri Lee (Undergraduate Student at Vanderbilt University):
at Laboratory of Prof. Dr. Ulf Wagner and Dr. Elisabeth Jäger

Lisa Pankewitz (Bachelor Student at Leipzig University):
at Laboratory of Prof. Dr. Jens Meiler, Brain Bender

Minh Ganther (Master Student at Leipzig University):
at Laboratory of Prof. Dr. Heidi Hamm and Ali I. Kaya

Tobias Haensch (Master Student at Leipzig University):
at Laboratory of Prof. Dr. Vsevolod V. Gurevich and Qiuyan Chen

Isabel Kratochvil
(Master Student at Leipzig University):
at Laboratory of Prof. Dr. John A McLean and Ewa Jurneczko

Hereby their short research and travel reports are listed… read more…

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Short Report by Dr. Wilma Neumann: BIOLOGICAL EVALUATION OF CARBORANE-CONTAINING COX INHIBITORS at Vanderbilt University (SEPT 09 – SEPT 19)

Monday, 2. November 2015 10:07 | Author:

WilmaNeumannWilma Neumann (postdoctoral associate at Leipzig University): Over the last years, the groups of Prof. Evamarie Hey-Hawkins (Institute of Inorganic Chemistry, Leipzig University) and Prof. Larry Marnett (Department of Biochemistry, Vanderbilt University School of Medicine) have developed a very fruitful collaboration, supported by the Leipzig-Vanderbilt collaboration project. Recently, we described a very potent and selective inhibitor of cyclooxygenase-2 (COX-2), a carborane-containing indomethacin analogue, which exhibits a novel binding mode in the COX enzyme pocket (W. Neumann, S. Xu, M. B. Sárosi, M. S. Scholz, B. C. Crews, K. Ghebreselasie, S. Banerjee, L. J. Marnett, E. Hey-Hawkins, ChemMedChem 2015, DOI: 10.1002/cmdc.201500199).

In September 2015, I returned to Vanderbilt to continue the biological evaluation of this promising inhibitor and conducted further experiments together with the very supportive co-workers of Larry Marnett. Besides the indomethacin derivatives, we evaluated a new class of carborane-containing COX inhibitors, extending our studies on the potential of the inorganic boron clusters which offer several unique properties for their application as pharmacophores. During my stay at Vanderbilt, I furthermore intensified our collaboration with Prof. Roger Cone (Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine), which also includes application of the versatile carborane clusters. After this inspiring week at Vanderbilt with insights into further techniques and new promising results, I presented the results of our collaborative research projects on carborane-containing COX inhibitors as well as the conjugation of COX inhibitors with platinum-based anti-tumour drugs at the Massachusetts Institute of Technology in Cambridge/MA.

My thanks go to the Leipzig-Vanderbilt collaboration project, supporting this as well as my former visits to Vanderbilt, which intensively promoted our collaborative projects with the Marnett group. I am also very thankful to Larry Marnett for hosting me again, and to his co-workers, especially Brenda Crews, Kebreab Ghebreselasie, Cristina Daniel and Philip Kingsley, performing the biological assays.

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Short Report of Gregory Sliwoski: STUDYING SMALL-MOLECULE MODULATORS OF THE Y4 RECEPTOR at University of Leipzig (AUG 20 – SEPT 10)

Monday, 19. October 2015 6:30 | Author:

by Gregory Sliwoski (doctoral student at Vanderbilt University): After completing my M.S. in pharmacology from Vanderbilt University under Prof. Dr. Jens Meiler in 2012, I joined Annette Beck-Sickinger’s lab at Leipzig University to further study the neuropeptide Y4 receptor (Y4R). This particular trip is my fourth stay in Leipzig in the collaboration between the labs of Prof. Dr. Jens Meiler (Vanderbilt) and Prof. Dr. Annette Beck-Sickinger (Leipzig). This collaboration combines computational modeling with cell-based signaling and mutategensis assays to characterize the activation of Y4R and discover new compounds that may enhance Y4R signaling. Signaling through this receptor has been shown to be a potential avenue for treatment of obesity and related diseases such as diabetes.

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Announcement: Scientific and Methode Modules “COMPUTATIONAL STRUCTURAL BIOLOGY WITH ROSETTA” at University of Leipzig

Thursday, 15. October 2015 10:19 | Author:

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Schedule
Module Computational Structure Biology Schedule 2015 (PDF, 102KB)

Registration
Please register until end of November by sending an e-mail to Anett Albrecht (albrecht@uni-leipzig.de).

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Short Report of Alyssa Loktis: PHYLOGENETIC APPROACH TO UNDERSTAND THE MECHANISM OF G PROTEIN ACTIVATION AND THE SELECTIVITY OF GPCR INTERACTION at University of Leipzig (MAY 04 – JULY 04)

Monday, 5. October 2015 6:08 | Author:

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by Alyssa Loktis (phd student at Vanderbilt University): I am a Neuroscience Ph.D. Candidate at Vanderbilt University in Nashville, TN, USA. I am jointly mentored in the laboratories of Dr. Heidi E. Hamm (pharmacology) and Dr. Jens Meiler (chemistry). I traveled to Leipzig, Germany to work with Dr. Peter Stadler in the Bioinformatics Department at the University of Leipzig. I lived in the Max Plank Guesthouse for 2 months while I completed my research collaboration.

Working primarily with a doctoral graduate student, Henrike Indrischek, in Dr. Stadler’s laboratory, I worked to reconstruct the evolutionary history of heterotrimeric G proteins through vertebrates and non-vertebrate chordates. Henrike has experience reconstructing and reconciling phylogenetic histories of another protein signaling family, arrestin. In addition, she has developed a computational method which assists in quickly and accurately annotating gene sequences from fragmented genomic data. This pipeline very effectively improved our analyses and aided in our collaboration efforts. Working with her was an excellent experience. Though I had very little background in genetics or bioinformatics, Henrike was able to instruct me on the finer points of gene annotation, sequence reconstruction, and phylogenetic analyses. We accomplished most of our objectives during my two month stay, and we continue to collaborate though I am back in Nashville.

Living in Leipzig for the summer was a fantastic experience. The members of the Bioinformatics Department, including Dr. Stadler and his laboratory, went out of their way to make me feel welcome and included in activities around the city. While in Leipzig, I attended the 1000 year anniversary celebration of the city as well as the annual city festival. I was also able to attend many cultural events such as outdoor concerts for the Bach festival, a performance of Die Valkyrie Opera during the Wagner festival, and several others. During my stay, the four-day Wave Gothic Festival also occurred. It was truly an active summer both academically and culturally.

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OFFERING RESEARCH INTERNSHIP – MAX KADE Scholarship for Students on Bachelor- or Master level: Call 2016

Wednesday, 30. September 2015 9:41 | Author:

vulu_web

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three‐four undergraduate students from each university to conduct a 10 week summer internship at the respective partner university. Travel costs up to $2000 and a weekly stipend of $400 are provided. They target rising junior and rising senior students at Vanderbilt University. To select the recipients of the stipend, they collect research project descriptions from around twenty core laboratories that support the collaboration between Vanderbilt and Leipzig Universities. Descriptions are provided by the graduate student or post‐doctoral mentor who will advise the undergraduate student. It is encouraged that the undergraduate student conducts research on the same or a related project in the laboratory of a collaborating investigator at their home institution during the academic year before or after their MKF internship.
________________________________________________________________
Offering Research Internship (until October 12)
The Group Leaders, PostDocs and PhD students can submit their internship offers, together with the project descriptions (Template) until October 12th. The exchange will be administrated by Carie Fortenberry (VU, c.fortenberry@vanderbilt.edu) and Anett Albrecht (LU, albrecht@uni-leipzig.de) including organization of housing and VISA. Please send your Research Internship offer to both e-mail addresses.

Download: Max-Kade Foundation grant proposal (Wordfile, 50KB)

Read more …

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Announcement: Scientific and Methode Modules “COMPUTATIONAL STRUCTURAL BIOLOGY WITH ROSETTA” at University of Leipzig

Tuesday, 29. September 2015 12:41 | Author:

JensMeiler

Date 07 – 18 December 2015
University of Leipzig, Fakulty for Bioscience, Pharmacy and Psychology, Institute of Biochemie, Brüderstraße 34, Seminar room 457 (4 floor), 04107 Leipzig

Responsible scientists
Prof. Dr. Jens Meiler (Vanderbilt University)
Prof. Dr. Annette Beck-Sickinger (Leipzig University)
Dr. Rene Meier (Leipzig University)

Aims
Teach theoretical and practical aspects of computational structural biology using the Rosetta software.

Basics
Biochemistry, amino acid structure, peptide bond, secondary structure, tertiary structure, small molecule binding, central dogma of molecular biology

Contents
Theoretical and practical aspects of protein sequence alignments, secondary structure prediction, comparative modeling, protein-protein and protein-ligand docking. Structure-based drug design, virtual screening. This course includes a laboratory section.

Methods
Computational Algorithms

Examination Final Written Exam and Report
Credit points 4 ETCS
Maximum number of participants 20 (open for Doctoral Students, PostDocs and Master Students)

Registration
Please register until end of November by sending an e-mail to Anett Albrecht (albrecht@uni-leipzig.de).

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Short Report of Jörg Schaarschmidt: GENERATION OF A FULL-LENGTH GPHR MODEL at Vanderbilt University (MAY 2 – MAY 21)

Tuesday, 29. September 2015 12:30 | Author:

by Jörg Schaarschmidt (postdoc at Leipzig University): After attending the conference on “Biomolecular Structure, Dynamics, and Function of Membrane Proteins” at the Vanderbilt University, gaining insights into the extended field of membrane proteins and about interesting new techniques like femtosecond cristallography, I spent three weeks at Prof. Jens Meiler’s Lab conceiving and testing a strategy for combining the previously generated homology models of the ligand binding domain and the transmembrane domain of the thyrotropin receptor into a full-length structural model. With the expertise and direct feedback of the Meiler lab members I was able to overcome several obstacles and ended up with a feasible strategy to pursue during my ongoing work in Leipzig. The established and deepened cooperation with the Meiler Lab is furthermore an important factor for the successful continuation of the project. Before finally returning to Leipzig I attended the “GPCR Structure, Function and Drug Discovery” conference in Cambridge and presented the first results of the project.

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Announcement: Lecture on “Structural analysis of protein complexes by mass spectrometry and computational modelling – basics and applications of a beautiful combination” at Vanderbilt

Wednesday, 3. June 2015 9:39 | Author:

25105_CMS_IMAGECenter for Structural Biology

Dr. Stefan Kalkhof, Post-Doc, Mass Spectrometry Group, Department of Proteomics, Helmholtz-Centre for Environmental Research – UFZ, Leipzig, Germany

Date
05 June 2015

Venue
Center for Structural Biology, 465 21st Ave South, BIOSCI/MRBIII, Vanderbilt University, Nashville, TN 37221

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Announcement: Course on “Site-specific protein labeling and incorporation of unnatural amino acids in live cells” at Vanderbilt University

Thursday, 23. April 2015 10:14 | Author:

Irene CoinSyllabus – CPBP 330 – Special Topics in Protein Biochemistry

Dr. Irene Coin, Leipzig University, Faculty of Life Sciences, Institute of Biochemistry

Date 14 – 21 July 2015
Venue Departments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, Room 5144B, Nashville, TN 37232-8725

The student will become familiar with modern techniques to incorporate labels and biophysical probes into intact proteins, including chemical synthesis, enzymatic modification, biorthogonal labeling and direct ribosomal incorporation of unnatural moieties.

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It is the sound of music – a remarkable partnership: Researchers from Leipzig and Nashville investigate molecular mechanisms

Tuesday, 24. March 2015 10:40 | Author:

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by Carsten Heckmann (LUMAG, 01-2015, 03/12/2015): To name the cities of Leipzig and Nashville in a sentence, could comes up with the idea: it could go on music. Classic here – Country there. But this is no topic for this research report. There is also the research field biochemistry – with a remarkable partnership between both universities.

To bridge 7,500 kilometer is not difficult for Prof. Dr. Annette Beck-Sickinger and Prof. Dr. Jens Meiler (Vanderbilt University). Sometimes they feel more like neighbors. “We work extremely well together,” says the Leipzig professor at the Institute of Biochemistry. More than 20 joint publications have appeared since 2008, emerged from around 30 joint projects. 40 PhD students have participated in the exchange program between Leipzig and Vanderbilt, not to mention various symposia and summer schools. Read more …

Leipzig University – Research Profile Areas
http://www.zv.uni-leipzig.de/en/research/research-profile-areas.html

LUMAG – Leipzig University
https://magazin.uni-leipzig.de/2015/03/einblicke-in-die-profilbereiche/

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Dicovering interaction mode of specific carbaborane derivatives with GPCRs at Vanderbilt University

Tuesday, 17. March 2015 14:45 | Author:

by Peter Schmidt (postdoc at Leipzig University): GPCRs have been shown to play a crucial role in a variety of diseases, such as obesity, metabolic diseases and cancer. Accordingly, they are an attractive target for the investigation of new pharmaceuticals. Di-carba-closo-dodecaboranes(12) (closo-C2B12H12 or carbaboranes) were recently established as pharmacophores. Their unique properties (hydrophobicity, three-dimensional aromaticity, etc.) make them interesting targets for drug development.

In this project, specific carbaborane derivatives will be prepared and used either as conjugates with peptides of the melanotropin (MSH) or neuropeptide Y (NPY) family, or as small molecules that impart their action via GPCRs to study and understand their influence on the selectivity and activity. NPY binds to four so-called Y-receptors, MSH to five so called MC-receptors that all belong to the family of GPCRs. Especially MC4, Y2 and Y4 receptors are of significance as they play an important role in metabolic diseases. Efforts to develop single-subtype specific ligands often suffer from unwanted affinity to other receptors with similar binding mode. We have already shown that modification of NPY analogs with carbaboranes or carbaborane analogs of nonsteroidal anti-inflammatory drugs (NSAIDs) can influence the selectivity and activity.

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Max-Kade Scholarship 2014: Short-Reports

Tuesday, 28. October 2014 15:03 | Author:

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2014 eight students on Bachelor- or Master level got the Max-Kade Scholarship to participate in ongoing research projects between Vanderbilt University and the University of Leipzig. Hereby there short research and travel reports:

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Announcement: Scientific and Methode Modules “Computational Structural Biology with ROSETTA”

Wednesday, 15. October 2014 10:59 | Author:

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Date 08 – 19 December 2014
Venue Biotechnologisch-Biomedizinisches Zentrum (BBZ), Seminar room 1.1, Deutscher Platz 5, 04103 Leipzig

Responsible scientists
Prof. Dr. Jens Meiler (Vanderbilt University)
Prof. Dr. Annette Beck-Sickinger (Leipzig University)
Dr. Jonathan Sheehan (Vanderbilt University)
Dr. Rene Meier (Leipzig University)

Aims
Teach theoretical and practical aspects of computational structural biology using the Rosetta software.

Basics
Biochemistry, amino acid structure, peptide bond, secondary structure, tertiary structure, small molecule binding, central dogma of molecular biology

Contents
Theoretical and practical aspects of protein sequence alignments, secondary structure prediction, comparative modeling, protein-protein and protein-ligand docking. Structure-based drug design, virtual screening. This course includes a laboratory section.

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Identification of new target GPCR at Vanderbilt University

Tuesday, 7. October 2014 12:56 | Author:

by Antje Brüser (postdoc at Leipzig University): I had the possibility to join Vanderbilt University from March 26th – July 11th 2014 as a visiting scholar to initiate a new project between our group (headed by Prof. Torsten Schöneberg) and the group of Prof. Larry Marnett. We focus in our lab on orphan G-protein coupled receptors (oGPCR) and the Marnett lab is working on COX inhibitors. Since years they have been interested in products of the COX-2 pathway and the identification of receptors which they bind to. In our new cooperation we have the knowledge and expertise to search and functional analysis of oGPCR. During my stay at the Marnett lab I could realize experiment for testing some GPCR and was able to identify new GPCR for prostaglandin glycerol-esters. These results now build the basis for a hopefully long and interesting cooperation.

Next to the very interesting work in the lab I did not only met nice people, I also had the possibility to discover parts of the US like Washington, DC, Memphis and New Orleans. I was very happy to join the group of Jens Meiler to their retreat to the Smoky Mountains for a weekend. Additionally, I had great evenings at Larry´s and Jens´ house and with the guys from the Marnett Lab. But the most awesome experience was the terrific southern American cuisine in combination with the very gorgeous people in Nashville.

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Biological evaluation of modified COX inhibitors at Vanderbilt University

Tuesday, 1. July 2014 7:25 | Author:

by Wilma Neumann (doctoral student at Leipzig University): After a successful start-up of a new collaboration project last autumn followed by a joint publication earlier this year, the group of Prof. Larry Marnett again welcomed me in their lab during my second research stay at Vanderbilt between May 21st and June 21st, 2014. Continuing our project on conjugates of cisplatin and COX inhibitors for use as antitumor agents, Brenda Crews, Cristina Daniel, and Kebreab Ghebreselasie actively supported me with the biological evaluation of new conjugates I had prepared in Leipzig. I am also very thankful to Cristina for introducing me to further molecular biology techniques and for her great effort performing numerous experiments within the short period of my stay. Besides cisplatin–COX inhibitor conjugates, we also continued with investigations on structure–activity relationships of carbaborane-containing COX inhibitors, our second collaboration project with the Marnett group in which we use borane clusters as pharmacophores to obtain novel COX inhibitors with improved inhibitory potency and selectivity. Further thanks go to Rosanne Delapp (Department of Civil and Environmental Engineering) for performing ICP-MS measurements of numerous cell samples.

Besides research, I enjoyed the hot and often quite humid weather, exploring the different districts and parks of Nashville and entering the fray at the country music festival (CMA Music Festival).

The research stay again yielded lots of results providing the basis for upcoming joint publications and enabled planning of the next steps within our collaboration projects. It also gave me further interesting insights into the Southern Culture. I gratefully acknowledge generous support from the Vanderbilt-Leipzig collaboration project and the bilateral project-related exchange program by the German Academic Exchange Service (DAAD-PPP-USA).

CMA-music-festival Cumberland-River view-of-Nashville

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Research stay at the Division of Nephrology and Hypertension at the Vanderbilt University Medical Center (VUMC)

Monday, 23. June 2014 13:34 | Author:

by Thomas Ebert (postdoc at Leipzig University): From January 3rd, 2014 – April 4th 2014, I had the opportunity to stay at the Division of Nephrology and Hypertension at the Vanderbilt University Medical Center (VUMC) in Nashville, Tennessee as a visiting scholar.

In our group in Leipzig (headed by Prof. Mathias Fasshauer), we focus on adipocyte-secreted proteins, so called adipokines, in renal dysfunction. To investigate adipokines in diabetic nephropathy (DN), a severe consequence of long-term diabetes mellitus, animal studies are vitally important. The group of Prof. Raymond C. Harris (Chief of the Division of Nephrology and Hypertension at VUMC) introduced the enos db/db-mouse as the currently best mouse model to study DN. Therefore, we were interested in their DN-prone mice and asked whether a short exchange would be possible. We appreciated the positive answer of Dr. Harris very much and in January 2014, I started my 3 months-trip to the “Music City” Nashville, TN. During the 3 months in Nashville, I was able to obtain knowledge in the enos db/db-mouse and learned basic techniques to study DN in rodents. Furthermore, Dr. Harris and his colleagues showed me how to investigate different renal endpoints. During the stay, I could even start working on some of our proposed projects in Leipzig.

Besides getting in touch with the lab work at Dr. Harris’ Division of Nephrology and Hypertension, I travelled a lot during the weekends and I was able to see many parts of „The Volunteer State“ Tennessee including Memphis, Knoxville, Chattanooga and the famous Great Smoky Mountains National Park. But Nashville is not just located in the middle of Tennessee but also just next to many other important cities of other States, such as Indianapolis, Louisville, Atlanta and Birmingham.

My experiences in Nashville will help me to set up experiments for our new projects in Leipzig within the IFB AdiposityDiseases. I gratefully appreciate the grant of the IFB supporting me during the research stay („MetaRot program“).

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Announcemnet: Summer School on CHEMICAL BIOLOGY OF GPCR LIGANDS

Saturday, 7. June 2014 12:56 | Author:

Prof. Dr. Annette G. Beck-Sickinger
(Leipzig University, Institute of Biochemistry)
21-28 July 2014, Vanderbilt University

Many ligands of GPCR belong to the family of peptides and proteins. This includes all class B GPCR ligands, e. g. calcitonin, glucagon and the pharmacologically relevant GLP-1, from which modified analogies have entered the anti-diabetic market recently (e. g. Exenentide, Liraglutide, etc.). But also in the family of class A GPCR different peptide and protein hormones (angiotensin, bradykinin, chemokines, FSH, etc.) play an important role in physiology.

Peptide ligands have some unique properties. They can be obtained by solid phase peptide synthesis and chemically modified with specific side-chain protection strategies. This includes selective labeling with fluorescent dyes, radioactive tracers, biotin, EPR- or NMR- probes to study their biophysical and structural properties. Furthermore, to overcome the proteolytic lability of peptides, modifications like lipidation or PEGylation have been successfully introduced to obtain drugs that are on the market. Protein ligands are usually produced recombinantly and accordingly modification are limited to the 20 proteinogenic amino acids by classical techniques. Novel methods like mis-charging of tRNA and chemical ligation strategies will be discussed that allow including non-natural amino acids into proteins. By using expressed protein ligation a combination of recombinant production and solid phase synthesis leads to protein with full segments replaced by solid phase synthesis and accordingly semi-synthetic protein production.

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Leipzig PhD use high performance computational ressources for protein modeling

Thursday, 27. March 2014 16:41 | Author:

by Tristan Zellmann (doctoral student at Leipzig University): I had the opportunity to stay at Jens Meilers lab in Nashville from 11/27/13 to 12/06/13 as well as from 01/27/14 to 03/22/14 to complete the modeling for an upcoming publication.

During my stay, I had the opportunity to take part in two great, american traditions – arriving the day just before Thanksgiving, I got invited to an actual Thanksgiving dinner, featuring delicious turkey, mashed potatoes, gravy, buttery carrots and even pumpkin pie. Another culinary and cultural highlight was a super bowl party complete with chili, buffalo wings and lots of snacks, even if the Seattle Seahawks seemed to dominate a bit too much. Besides these great examples of southern hospitality, Nashville itself welcomed me with untypically cold weather. On the warmer days, the beautiful centennial park was great for a walk. Being called “Music City, USA”, Nashvilles reputation for a vibrant music scene is also no understatement – on most nights, you can just randomly go into a bar for some great live music, even besides the country music Nashville is said to be famous for.

The beautiful campus of the Vanderbilt University, the cultural offerings of Nashville and the good atmosphere at the lab all made my trip very much worth it. I am glad I had the chance to experience the welcoming, open attitude of the people in the southern US.

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Research Stay at Vanderbilt University on Arrestin Proteins

Thursday, 13. March 2014 10:24 | Author:

by Lizzy Wanka (doctoral student at Leipzig University): In the autumn of 2013, I had the great opportunity to visit Prof. Dr. Vsevolod V. Gurevich`s lab at the Vanderbilt university for 12 weeks. Prof. Gurevich and his group work with arrestin proteins and investigate among others their binding to receptors and other signaling proteins. I wanted to study the binding of different arrestin mutants to NPY receptors and NPY receptor chimera. Together with Dr. Luis E. D. Gimenez, I performed various bioluminescence resonance energy transfer assays and learned a lot about the arrestin recruitment and the optimization of this assay. The discussions about analyzing the data were revealing and very helpful. I enjoyed the work in this lab and the great atmosphere as well as the activities with my colleagues.

The most interesting experience during my stay was Halloween and the preparation for this day. I learned a lot about the American way of life and made some close friendships. All in all, I savored my stay in the country music city Nashville.

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Max-Kade Foundation Partners with VU-LU Collaboration

Friday, 31. January 2014 4:44 | Author:

The Vanderbilt-Leipzig Collaboration is pleased to announce its partnership with the Max Kade Foundation to support the exchange of students and faculty between the universities. The Max Kade Foundation takes pride in participating and sponsoring various programs which encourage the exchange of academic ideas among universities and colleges in the United States and in German-speaking countries (www.maxkadefoundation.org). The foundation recently awarded the Vanderbilt-Leipzig research exchange program a grant to cover the travel and support of six undergraduate student exchanges, as well as two faculty exchanges per year. The students will travel to their respective university and join a research project under a senior supervisor. They will be engaged for ten weeks in the challenging and exciting scientific communities of each institution, while likewise being immersed in rich cultural experiences. During their exchange, the two faculty members will teach short courses which will introduce new technologies and research endeavors not available at the other university.

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Leipzig Post-Doctoral Fellow in Nashville / Vanderbilt University

Friday, 20. December 2013 10:28 | Author:

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by Stephan Theisgen (Post Doc at Leipzig University): From 16th September to 14th October I had the opportunity to visit Prof. Jens Meiler’s Lab where I wanted to perform some structural computations to complete a pending scientific paper. This turned out to be a major task for 4 weeks only. But together with Dr. David Nanneman, Stephanie Hirst DeLuca and Sam DeLuca, we managed to finish this ambitious project successfully. In the process, I learned a lot about computational structural biology and became familiar with an important method in this field.
Beside the heavy scientific work, I enjoyed very much the special southern cuisine, visiting downtown Nashville and of course some country music places. After some self-studies of football rules and strategies, I decided to watch a football game (Vanderbilt Commodores vs. Missouri Tigers) in the Vanderbilt Arena. Even though the Vandi team lost the game, I enjoyed the atmosphere a lot.
Before I had to return to Germany, Jens Meiler invited us to a picnic at a beautiful winery. The wine was very good and the weather, especially compared to Germany, fantastic: 27 °C and sunshine.

To sum it up: a busy but very successful scientific exchange, where I learned a lot from very nice colleagues and discovered a beautiful part of the USA.

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Vanderbilt, Leipzig research collaboration sees strong results

Tuesday, 19. November 2013 13:03 | Author:

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by Matt Windsor: During the past two weeks, the fifth international symposium between Leipzig and Vanderbilt universities was held on the Vanderbilt campus, as seven Leipzig faculty members presented a series of chemical biology-related lectures. The symposium offered an opportunity to highlight the ongoing success of the international collaboration. To date, there have been 17 joint publications between research groups at the two universities.

This summer, the first federally funded research grant resulting from the collaboration was awarded by the National Institutes of Health (NIH). The project is aimed at developing drugs for a specific type of protein known as a G protein-coupled receptor (GPCR). The grant was spearheaded by Annette Beck-Sickinger, Ph.D., professor of Biochemistry and Bioorganic Chemistry at Leipzig, and at Vanderbilt by Jens Meiler, Ph.D., associate professor of Chemistry and Pharmacology, and David Weaver, Ph.D., assistant professor of Pharmacology and scientific director of the Vanderbilt High Throughput Screening Facility.

“We work on GPCRs that play a role in obesity and colon cancer,” Beck-Sickinger said. “We know these receptors. In Leipzig, we have set up biochemical assays that are necessary to study these receptors, a process that required several years of dedicated effort.”

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Workshop at VU: Mass Spectrometry based methods for protein structure analysis

Tuesday, 19. November 2013 12:42 | Author:

by Dr. Stefan Kalkhof, Prof. Dr. Martin von Bergen and Prof. Dr. John McLean: In an one-week curse 30 PhD students and postdocs of the Vanderbilt University were introduced to modern mass spectrometry methods and recent technical developments. The participants were made familiar with the theory of H/D exchange, ion mobility, chemical crosslinking, and labeling techniques using chemicals or radicals. Furthermore, to give the participants a better feeling concerning the potential as well as advantages, experimental challenges, and limitations many own projects and experimental protocols were presented and critically discussed. Finally, a session was used to discuss how one could practically utilize the techniques to answer current questions of participant`s projects.

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At this point we especially would like to thank all participants for their great interest in the topics and the very active participation during the whole curse as well as Prof. Dr. Jens Meiler for the perfect organization.

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Vanderbilt Undergraduate Student in Leipzig

Tuesday, 19. November 2013 12:24 | Author:

Hannah Berke

by Hannah Berke (undergraduate student at Vanderbilt University): My two months spent in Leipzig were a wonderful experience. The work in the lab was informative and enjoyable and living in Leipzig changed my life. My stay in Leipzig was extremely fun and gave me the opportunity to travel and experience Europe and Germany specifically. The city itself is a perfect size, very similar to Nashville, and I lived in an area that was about a 5 minute tram ride or 20 minute walk from the city center, or “Mitte,” that is full of shops and restaurants. The abundance of parks and cafes made it easy to spend time outside – barbecuing, reading, and relaxing with friends.

I was the only American undergraduate working in the lab that semester, which was challenging at first, but extremely rewarding. I made friends with my roommate and became very close with a group of German students at the Universität Leipzig. They showed me all around the city, both during the day and at night, and I travelled with some to their hometown of Hannover one weekend. Getting around in a country where I didn’t know the language was difficult, but having friends that spoke the language helped me enormously. There was never a shortage of things to do in Leipzig, and if there wasn’t a musical event or café open, I would just walk around the city and visit historical sites like the Völkerschlachtdenkmal, Thomaskirche, and the huge Clara-Zetkin Park.

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DAAD RISE Program: Call 2014

Tuesday, 22. October 2013 12:04 | Author:

The German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) announced a new call for its program “Research Internships in Science and Engineering” (RISE) for summer internship in 2014.

The program works in both ways. German and American Ph.D. students submit internship offers for undergraduates students for a duration of 8 to 12 weeks. German bachelor students can work as research interns at U.S. universities and U.S. undergraduates can work in labs of German universities. The DAAD provides stipends to help cover living expenses, the partner universities and research institutes provide housing assistance. The call is applicable for all fields of biology, chemistry, physics, earth sciences and engineering.

Please visit for further information:

DAAD RISE DAAD RISE worldwide
To promote the exchange of students from North America and Great Britain to Germany in the fields of natural science, engineering and life sciences To enable young German scientists to conduct research abroad
Offering Research Internship Offering Research Internship
October 1, 2013 – November 30, 2013 October 7, 2013 – November 24, 2013
Apply for Research Internship Apply for Research Internship
December 6, 2013 – Januar 31, 2014 December 2, 2013 – Januar 12, 2014











Students and researchers from Leipzig and Vanderbilt Universities with an interest in exchange activities with the other partner university are kindly asked to contact Mrs. Anett Albrecht for more information and assistance.

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New Grant supports joint Research Project between Vanderbilt and Leipzig Universities

Wednesday, 4. September 2013 13:30 | Author:

Annette Beck-Sickinger and Torsten Schoeneberg from Leipzig University and Jens Meiler from Vanderbilt University receive a grant “Ensemble Docking Interrogates Structural Determinants of Ligand-Protein Interactions” that is jointly funded by the National Science Foundation (NSF) and the Deutsche Forschungsgemeinschaft (DFG). The grant will develop a novel docking algorithm that leverages Structure-Activity-Relations (SAR) and apply this algorithm to understand regulation of G-Protein Coupled Receptors (GPCRs).

Project Summary

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4th Vanderbilt-Leipzig Cooperation Workshop

Tuesday, 6. August 2013 13:38 | Author:

The 4th international scientific symposium was held with members of the Leipzig University and Vanderbilt University from the 6th to the 12th of July 2013 in Leipzig. The focus of this event taking place at the Biotechnological-Biomedical Center (BBZ) of the University of Leipzig was the further organization and consolidation of the university partnership (MOU) between Leipzig and Vanderbilt University. Likewise this event was set out to be a Kick-Off-Meeting for two new international junior research groups, which are funded by the State Ministry of Science and Arts through the European Social Fond (ESF) since July 1st.

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Leipzig Post-Doctoral Fellow in Nashville / Vanderbilt University

Tuesday, 6. August 2013 12:40 | Author:

by Menyhárt B. Sárosi (Post-Doctoral Fellow): In the spring of 2013, I had the opportunity to visit the laboratory of Terry Lybrand, professor of Chemistry and Pharmacology at Vanderbilt University. His group has extensive experience in computational studies involving ligand complexes with cyclooxygenase (COX) enzymes, among several other key areas of research.

During the one month stay I became familiar with Amber12 and AmberTools12 programs with the scope of using them in a project involving carbaborane-containing cyclooxygenase (COX) inhibitors. More specifically, the target is to use computational methods to design potential carbaborane-containing inhibitors and to gain insight into their interactions with the COX system. After learning the essentials of the above mentioned codes and how to visualize and manipulate the structure files of large biomolecules, I have been able to prepare input files and run several test QM/MM calculations on one of the target systems. The subsequent steps of this project will be carried out in Leipzig, in a close collaboration with Prof. Lybrand and his research group at Vanderbilt University. Besides Prof. Lybrand, Jonathan Sheehan and Chris Moth have also been very helpful in explaining the above mentioned computational techniques and in giving advice concerning the computational analysis of COX inhibition. I also had the pleasure of meeting associate professor Jens Meiler and members of his research group.

The financial support for my research visit has been provided from the Bilateral Program for Cooperative Research Grants (DAAD-PPP-USA 2013) and from the Vanderbilt-Leipzig collaboration funds.

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Symposium and New Grants Bolster Vanderbilt-Leipzig Partnership

Sunday, 7. July 2013 21:51 | Author:

Two new grants and a summer symposium with workshops highlight ongoing collaboration efforts between Vanderbilt University and Leipzig University:

1) Vanderbilt University received a grant from the Max Kade Foundation in support of an undergraduate and graduate student exchange program in collaboration with Leipzig University. The collaboration is spearheaded by Drs. Jens Meiler (Vanderbilt University) and Annette Beck-Sickinger (Leipzig University).

2) The Meiler and Weaver laboratories at Vanderbilt University received a grant from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK, NIH) for the development of “Small Molecule Probes to Investigate Structure and Function of Y Receptors.” This project is a collaboration with Annette Beck-Sickinger at Leipzig University, Germany who is an expert on Y-receptor biochemistry. The project is the first federally funded chemical biology research grant resulting from Vanderbilt and Leipzig Universities collaboration. Project Summary and Press Release

3) This July 6th through July 12th researchers from Vanderbilt and Leipzig University will meet in Leipzig for the 4th annual partnership symposium and scientific workshop. Drs. Gurevich, Lybrand, Mchaourab, and Meiler from Vanderbilt University will participate in scientific presentations and meet faculty from Leipzig University. The Memorandum of Understanding between the two institutions will be renewed, and faculty will discuss the strategic objectives of the collaboration. Press Release

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Announcement: Summer School on Molecular Modeling

Monday, 10. June 2013 9:19 | Author:

Prof. Dr. Terry Lybrand, Vanderbilt University Nashville, USA

10. – 12. July 2013, Leipzig University

Prof. Dr. Terry Lybrand (Vanderbilt University)

Course description: This short course will cover basic background information and applications details for molecular mechanics calculations, including energy minimization and molecular dynamics simulations. Some basic theory will be presented, but the course will focus primarily on practical details for molecular mechanics calculations and will include numerous “hands-on” exercises, i.e., the students will get the opportunity to perform all necessary steps for typical calculations. We will discuss the capabilities and limitations of different types of calculations, the information required to perform useful calculations, and analysis techniques to allow you to extract useful information from these calculations. Most exercises will involve calculations for large biological molecules, but the methods presented are suitable for a wide range of chemistry applications.

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Announcement: Summer School on EPR Spectroscopy

Monday, 10. June 2013 9:11 | Author:

15. – 18. July 2013, Leipzig University
Prof. Dr. Hassane Mchaourab, Vanderbilt University Nashville, USA

Hassane Mchaourab (Vanderbilt University)

Course description: This is an advanced, intensive course directed at graduate students. It is intended to provide an overview of the EPR spectroscopy and its application to protein biophysics. Electron paramagnetic resonance is a magnetic resonance technique for the analysis of naturally occurring or introduced free radicals. In protein spin labeling, a stable free radical, spin label, is introduced specifically into the protein sequence via site-directed mutagenesis. The lectures will describe the technical and biochemical aspects of protein spin labeling, discuss the spectral parameters that are determined from EPR analysis of spin labeled proteins and their structural and dynamic interpretation. The lectures will end with an examination.

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The Leipzig Connection

Wednesday, 9. January 2013 12:55 | Author:

by David Salisbury | posted at news.vanderbilt.edu

Leipzig master students Philine Hietschold and Franziska Dreher (sitting) present their protein models to Vanderbilt graduate students Greg Sliwoski and Liz Dong during their 2012 summer internship at Vanderbilt University in the Meiler laboratory. (Courtesy of Jens Meiler)

In 2007, while Jens Meiler was visiting his parents in Germany, the associate professor of chemistry was invited to give a lecture at his alma mater, the University of Leipzig.

Jens Meiler (John Russell / Vanderbilt)

“When I gave that talk on my research in structural and chemical biology, I found a tremendous amount of interest in what we are doing at Vanderbilt and learned that there is a great deal of complementary research going on in Leipzig,” Meiler said.

In fact, Meiler stirred up so much interest that two years later Annette Beck-Sickinger, professor of biochemistry and bioorganic chemistry at Leipzig, spent her sabbatical at Vanderbilt. During her visit she helped establish a number of collaborations, leading the administrations of the two universities to sign a five-year memorandum of understanding that allows and encourages academic exchanges, facilitates joint research programs, student programs and a cultural exchange program.

Over the last few years, the size of the collaboration has grown to embrace 20 faculty members at the two universities; more than 20 graduate students have spent time studying at the other campus; and groups of five undergraduates have been exchanged for the last few summers. Last October, Leipzig Professor Daniel Huster spent 10 days on campus to teach a mini-course on the use of NMR spectroscopy in biology.

“Leipzig has become one of our half-dozen strategic international partners,” said Tim McNamara, vice provost for faculty and international affairs. “It is a very productive relationship and we certainly want it to prosper.”

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DAAD RISE Program: Call 2013

Friday, 12. October 2012 17:03 | Author:

The German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) announced a new call for its program “Research Internships in Science and Engineering” (RISE) for summer internship in 2013.

The program works in both ways. German and American Ph.D. students submit internship offers for undergraduates students for a duration of 8 to 12 weeks. German bachelor students can work as research interns at U.S. universities and U.S. undergraduates can work in labs of German universities. The DAAD provides stipends to help cover living expenses, the partner universities and research institutes provide housing assistance. The call is applicable for all fields of biology, chemistry, physics, earth sciences and engineering.

Please visit for further information:

DAAD RISE DAAD RISE weltweit
To promote the exchange of students from North America and Great Britain to Germany in the fields of natural science, engineering and life sciences To enable young German scientists to conduct research abroad
Offering Research Internship Offering Research Internship
October 1, 2012 – November 30, 2012 October 8, 2012 – November 25, 2012
Apply for Research Internship Apply for Research Internship
December 6, 2012 – Januar 31, 2013 December 3, 2012 – Januar 13, 2013











Students and researchers from Leipzig and Vanderbilt Universities with an interest in exchange activities with the other partner university are kindly asked to contact Mrs. Anett Albrecht for more information and assistance.

Category:Research, Students | Comment (0)

My Summer in Leipzig – The Universität Leipzig/VU Collaboration

Wednesday, 5. September 2012 17:51 | Author:

by Shilpa Mokshagundam (Vanderbilt University, Class of 2014)

My internship took off very  quickly! Within the first day, my PhD supervisor Tom explained to me the basis of our project and allowed me to assist in preparing and running an agarose gel. This was quite different from any American internship, where the intern is usually required to conduct a literature review for at least 1-2 weeks.

The next week, I travelled with the AGBS to Krummenhennersdorf for a week-long seminar retreat. During the morning and afternoon, PhD students presented their current work. In the evenings, we hiked, cooked dinner and sang songs around the campfire. Tom and I officially began our project the next week and worked on inserting Delta-like-Kinase-1 (DLK1) into our vectors of interest. Tom taught me how to run PCR and perform digestion, ligation and transformation.

As I began to get acclimated to living in Germany, I started to explore the wonderful city of Leipzig. I experienced BachFest, a 10-day music festival that takes place in Leipzig city center. I also explored the Museum der bildenden Künste Leipzig on one of the “free admission days”. I also had the pleasure of going paintballing with the PhD students in AGBS. I was so happy to see the lab group bonding and spending time together outside of work. I think this allowed for a friendly and trusting working atmosphere and I truly admire the tight friendships within AGBS.

Over the next weeks, I worked with Tom to express our protein and began protein purification. These procedures were completely new to me but Tom was very encouraging and helped me through every step of the process! At this time, I travelled with my fellow RISE interns to the Dresden for the RISE conference. We explored the beautiful city and discussed opportunities for studying in Germany after completing our Bachelor’s degree.

After returning from the RISE conference, I began to run chitin columns with hopes of obtaining the purified protein. The last step, which was performed during my last week in Leipzig, was using SDS-PAGE to analyze the success of the protein purification method. Luckily, Tom and I found the method to be quite successful! My last week in Leipzig was bittersweetI had formed such tight relationships with my fellow RISE students, my PhD supervisor Tom and the Masters and PhD students in AGBS and it was difficult saying goodbye to everyone!

I am so grateful to Tom, the RISE program, Dr. Annette Beck-Sickinger, Dr. Jens Meiler, the entire AGBS and the Leipzig/VU collaboration for helping in this experience. I feel so lucky to have been blessed with this incredible opportunity and I hope to motivate more students to study in Leipzig!

Picture: Shilpa and a friend at the Art Museum (“Bildermuseum”) in Leipzig

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Announcement: Summer School on Receptor Theory and GPCR signalling via G proteins and arrestins

Tuesday, 28. August 2012 15:45 | Author:

Prof. Dr. Vsevolod Gurevich, Vanderbilt University, Nashville, TN

8 – 12 October 2012, Leipzig University

Arrestins are proteins which regulate the signalling and trafficking of hundreds of different G-protein-coupled receptors (GPCR). But arrestins also interact with other classes of membrane receptors and over 20 diverse types of soluble signalling protein. Arrestins can block GPCR coupling to G proteins in two ways. But they can also modify gene expression by increasing the transcription of certain genes.Several arrestin-binding partners play a key role in the signalling pathways regulating cell proliferation, survival and apoptosis. Since multiple genetic disorders can be associated with excessive signalling by mutant G-protein-coupled receptors, arrestin is an interesting target for therapeutic intervention. Vsevolod Gurevich uses different approaches for the investigation of arrestin functions ranging from basic biochemistry and X-ray crystallography to cell culture and transgenic animals. In his lectures, he will give an overview of receptor theory, explain key methods of studying receptors and receptor-initiated cell signalling as well as explain key signalling pathways initiated by GPCRs via activation of G proteins and arrestins.

If you want to attend the summer school, please register by sending an e-mail to Anja Pohl.

Further information (pdf): Schedule of Summer School on Receptor Theory

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Announcement: Summer School on Solid-State NMR Spectroscopy in Biology

Monday, 13. August 2012 15:55 | Author:

Prof. Dr. Daniel Huster, University of Leipzig

2 -11 October 2012, Vanderbilt University, Nashville, TN

Due to the success of NMR studies on soluble molecules the original NMR spectroscopy on solid materials such as crystals, glasses, powders and polymers has long played an inferior role. However, in the last decade, solid-state NMR spectroscopy has widely been applied to complicated biological questions such as the study of aggregated and amyloidic protein states, membrane proteins, biological tissue or other molecular aggregates of large molecular mass. As there is no principle limit on the molecular mass of the investigated molecules and the fact that the resolution and sensitivity of the technique seems to constantly improve, solid-state NMR is seen to have a bright future in structural biology and biophysics, but also in medical research. In the summer school, the basic principles of solid-state NMR in comparison to solution NMR will be highlighted in a general way including practical exercises in modelling line shapes and NMR spectra on the computer. Further, current examples on the structure elucidation and dynamic characterization of amyloids and membrane proteins will be discussed.

Recommended Literature:

Baldus, M. (2002) Correlation experiments for assignment ans structure elucidation of immobilized polypeptides under magic angle spinning. Prog Nucl Magn Reson Spectrosc 41: 1-47.

Huster, D. (2005) Investigations of the structure and dynamics of membrane-associated peptides by magic angle spinning NMR. Prog Nucl Magn Reson Spectrosc 46: 79-107.

Hong, M. (2007) Structure, topology, and dynamics of membrane peptides and proteins from solid-state NMR Spectroscopy. J Phys Chem B 111: 10340-10351.

More information will be available beginning of September.

Category:Events, Research, Students | Comment (0)

Vanderbilt PhD students in Leipzig

Wednesday, 26. October 2011 13:48 | Author:

Stephanie and Liz are graduate students in the Meiler Laboratory at Vanderbilt University.  They both work on the computational investigation of GPCRs and their interactions with various ligands. They recently took the opportunity to do a month-long research internship at Leipzig University in conjunction with their collaborators, who perform the wet-lab experiments associated with the proteins they model. Herein, they reflect on their experiences.

Liz Dong: For 4 weeks, I worked with the lab of Dr. Torsten Schöneberg on a project involving P2Y12, a receptor involved in platelet aggregation. My part of the project was to build a model of P2Y12 using Rosetta and dock known ligands into the model to assess their binding modes. While in the lab, I had the opportunity to meet and work with the students involved in the project. Maxi Cöster, Kay Simon and Philipp Schmidt were extremely helpful in explaining the P2Y12 system to me and the assays that are used to evaluate functionality of the receptor. I even had the opportunity to work in the wet lab to assist in data collection in between running computational jobs. During my 4 weeks, I was able to build models of P2Y12 and create docking poses of the ligands we are interested in studying, which will contribute nicely to a paper we are looking to submit soon.

Stephanie DeLuca: I was in Germany for five weeks.  During the first week, I attended the summer school of the SFB 610 graduate program in Dessau, where I got to know several graduate students from both Leipzig and Halle Universities, as well as the interesting research they are doing.  One thing that surprised me is the diversity of both systems being studied and methods being used.  The following week, I joined the Beck-Sickinger lab group at their external seminar in Krummenhennersdorf.  Because everyone in the lab gave a full scientific talk, I was able to get a good idea of what kind of research Prof. Dr. Beck-Sickinger’s group performs, as well as learn how they approach biomedically relevant problems and what kind of experimental techniques they use.  I spent my last three weeks working in the Beck-Sickinger lab alongside Daniel Rathmann.  The goal of my visit was to learn about the experiments used to study the prolactin releasing peptide (PrRP) and its receptor.  In addition to the IP accumulation assay often used in this lab, I also observed how to do solid-state peptide synthesis (SSPS) and fluorescence microscopy.  We managed to successfully obtain concentration-response curves that we can then use to assist/validate the computational modeling of this system.

Because we stayed in a guesthouse near the city center, there were always many exciting activities to enjoy while we were not in the lab. In addition to shopping and visiting the Bach, Stasi, and Grassi museums, we saw great performances by the Gewandhaus Orchestra and the Thomanerchor. We also traveled to Dresden and Berlin, where we learned more about German history.  While in Berlin, we visited many sites relevant to World War II and the Cold War, and we celebrated the German Reunification in front of the Brandenburg Gate.

The collaborative research centre SFB 610 “Variation in Protein Conformation: Cellbiological and Pathological Relevance” supported the research stay of Liz and Stephanie with a short-term-stipend.

Category:Other projects, Research, Students | Comment (0)