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BioAnalysis

Das Modul vertieft die Kenntnisse der Massenspektrometrie, der NMR-Spektroskopie und der Molekularen Modellierung ein. Das dreitägige Blockseminar findet vom 20. Mai bis 22. Mai 2015 in Leipzig statt.  

MADLI MS / ESI MS / NMR an Kohlehydraten (Dr. Jürgen Schiller)

Ablaufplan

20. Mai 2015 
09:00 – 10:00
Short Introduction: What will we do at Wednesday?
10:15 – 11:15
Benefits and drawbacks of NMR spectroscopic characterization of GAG polysaccharides (Practical Course)
11:15 – 12:15
Lunch
12:15 – 16:00
Conversion of GAG oligosaccharides into ammonia salts (Practical   Course)
16:00 – 17:00
Discussion of the achievements of the first day

21. Mai 2015 
09:00 – 10:00
 Short Introduction: What will we do at Thursday?
10:15 – 11:15
 How can the line-width of NMR resonances be influenced? (Practical Course)
11:15 – 12:15
 Lunch
12:15 – 15:30
 The impact of different MALDI matrices on the spectral quality (Practical Course)
15:30 – 16:00
 Discussion of the achievements of the second day

23. Mai 2015 
09:00 – 10:00
 Short Introduction: What will we do at Friday?
10:15 – 11:15
 Automated data analysis of the NMR spectra of complex mixtures (Practical Course)
11:15 – 12:15
 Lunch
12:15 – 16:00
 "DOSY" NMR helps to assign complex polysaccharides (Practical Course)
16:00 – 17:00
 Final discussion

June 2015 
 Report or written exam

 

Untersuchung der GAG-Protein-Wechselwirkung mittels Lösungs-NMR-Spektroskopie
(Prof. Dr. Daniel Huster, Georg Künze)

Ablaufplan

20. Mai 2015 
09:00 – 11: 15
Short introduction to NMR spectroscopy:
-    How the spectrometer works
-    Classical and quantum mechanical description of NMR
-    Product operator formalism
-    Two dimensional NMR spectroscopy
11:15 – 12:15
Lunch
12:15 – 15:30
Explore the GAG binding site of IL-8:
-    The HSQC experiment
-    Chemical shift perturbation experiments
15:30 – 17:00
Discussion of the achievements of the first day

21. Mai 2015 
09:00 – 11:15
NMR resonance assignment of ubiquitin (I):
-    Introduction to three dimensional correlation experiments
-    Setting up an HNCO experiment
11:15 – 12:15
 Lunch
12:15 – 15:30
 NMR resonance assignment of ubiquitin (II):
-    Spectrum processing
-    Sequential peak assignment

23. Mai 2015 
09:00 – 11:15
Study of ligand binding epitopes by STD NMR spectroscopy
11:15 – 12:15
 Lunch
12:15 – 15:30
 Determination of ligand binding affinities by STD NMR spectroscopy
15:30 – 17:00
 Final discussion

June 2015 
 Report or written exam
 

Ort:
[1] Institut für Medizinische Physik und Biophysik, Härtelstraße 16-18, 04107 Leipzig


Organisatorisches:
Maximale Teilnehmerzahl: 3-4 pro Kurs
Credit points: 2 (benotet)

Anmeldung:
Die Anmeldung erfolgt bis 15. Mai 2015 per e-Mail an Anett Albrecht.