We newly published a paper in ACS Applied BioMaterials on the synthesis and application of agarose polymers with pH-sensitive fluorescent dyes. We show the covalent end-on coupling of pH-sensitive dyes to agarose polymers and the usage of this agarose in monitoring pH changes of microbial cultures of photoautotroph and chemoheterotroph species incorporated within agarose hydrogels due to their metabolic activity.
Category: Publication
New publication about the detection of sulfonamide antibiotics
Our research group ‘Active Materials’ recently published an article on the detection of sulfonamide antibiotics with optical biosensors in the journal ACS Applied Materials & Interfaces. The technology developed in the research group is based on elastic hydrogel microparticles, so-called soft colloidal probes (SCP).
Sulphonamide antibiotics were the first synthetic antibiotics on the market and still have a a broad field of applications. Their extensive usage, wrong disposal, and limited degradation technologies in wastewater treatment plants lead to high concentrations in the environment, resulting in a negative impact on ecosystems and an acceleration of antibiotic resistance. Although lab-based analytical methods allow for sulfonamide detection, comprehensive monitoring is hampered by the nonavailability of on-site, inexpensive sensing technologies.
The publication shows a proof-of-concept for the detection of sulfonamide antibiotics in aqueous samples for application options in environmental monitoring. The presented technology exploit functionalized elastic hydrogel microparticles and their ability to easily deform upon specific binding with enzyme-coated surfaces to establish the groundwork of a biosensing assay for the fast and straightforward detection of sulfonamide antibiotics outside the laboratory.
New Paper on Modulating Paracrine Cell Signals in a Biomimetic Wound Healing Model
In cooperation with colleagues from Leibniz Institute of Polymer Research Dresden and Max Bergmann Center of Biomaterials Dresden, we published a paper in Gels on a biomimetic wound healing model using cocultures of primary human fibroblasts and macrophages in 3D collagen networks functionalized with sulfated glycosaminoglycans. We show that the network functionalization and the degree of sulfation of GAGs influences the paracrine cell-cell signaling in the in vitro model.
Something’s happening, Germany! Who will ensure less waste?
For the ZDF production “plan b” 2 employees went on the in search of the garbage visionaries. Their goal: To live more resource-consciously and create less waste. For this purpose visited the research laboratory of Dr. Christian Sonnendecker.
You can watch the entire report here: ZDF contribution
Evaluating scaling of capillary photo-biofilm reactors for high cell density cultivation of mixed trophies artificial microbial consortia
Capillary biofilm reactors (CBRs) are attractive for growing photoautotrophic bacteria as they allow high cell-density cultivation. Here, we evaluated the CBR system’s suitability to grow an artificial consortium composed of Synechocystis sp. PCC 6803 and Pseudomonas sp. VBL120. The impact of reactor material, flow rate, pH, O2, and medium composition on biomass development and long-term biofilm stability at different reactor scales was studied. Silicone was superior over other materials like glass or PVC due to its excellent O2 permeability.
Access the full article here:
https://onlinelibrary.wiley.com/doi/10.1002/elsc.202300014
Entropic repulsion of cholesterol-containing layers counteracts bioadhesion
Based on a cooperation with colleagues from Dresden, we newly published a paper in Nature on a new mechanism counteracting bioadhesion by entropic repulsion of orientational fluctuations in cholesterol multilayers. Our results not only reveal this new mechanism of entropic repulsion to be relevant in the surface properties of Collembola, but, also shows the high specificity of the cholesterol chemistry.
You can access the article here:
https://www.nature.com/articles/s41586-023-06033-4
Integrated electrosynthesis and biosynthesis for the production of adipic acid from lignin-derived phenols
To read the article by Dr. Rohan Karande, Alexander Franz, Prof. Dr. Falk Harnisch and Micjel Chávez Morejón click here: https://pubs.rsc.org/en/content/articlelanding/2023/gc/d3gc01105d
Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles
Click here for the article by Prof. Dr. Tilo Pompe, Veronika Riedl, Matthias Portius, Lara Heiser, Philipp Riedl, Torsten Jakob, Rosa Gehring and Thorsten Berg:
https://research.uni-leipzig.de/bact/wp-content/uploads/2023/05/2023-JMatChemB-SCP-SMX.pdf
Read more: Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles
Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains
Click here for the article by Dr. Henri Franquelim: https://www.cell.com/biophysj/fulltext/S0006-3495(23)00135-2
Biomaterial approaches for engineering and analyzing structure and metabolic states of microbial consortia within biofilms
Click here for the article by Prof. Dr. Tilo Pompe and Matthias Portius and Christian Dannenberg:
https://www.sciencedirect.com/science/article/pii/S0958166923000265?dgcid=author
New publication about the detection of sulfonamide antibiotics
Our research group ‘Active Materials’ recently published an article on the detection of sulfonamide antibiotics with optical biosensors in the journal ACS Applied Materials & Interfaces. The technology developed in the research group is based on elastic hydrogel microparticles, so-called soft colloidal probes (SCP).
Sulphonamide antibiotics were the first synthetic antibiotics on the market and still have a a broad field of applications. Their extensive usage, wrong disposal, and limited degradation technologies in wastewater treatment plants lead to high concentrations in the environment, resulting in a negative impact on ecosystems and an acceleration of antibiotic resistance. Although lab-based analytical methods allow for sulfonamide detection, comprehensive monitoring is hampered by the nonavailability of on-site, inexpensive sensing technologies.
The publication shows a proof-of-concept for the detection of sulfonamide antibiotics in aqueous samples for application options in environmental monitoring. The presented technology exploit functionalized elastic hydrogel microparticles and their ability to easily deform upon specific binding with enzyme-coated surfaces to establish the groundwork of a biosensing assay for the fast and straightforward detection of sulfonamide antibiotics outside the laboratory.
New Paper on Modulating Paracrine Cell Signals in a Biomimetic Wound Healing Model
In cooperation with colleagues from Leibniz Institute of Polymer Research Dresden and Max Bergmann Center of Biomaterials Dresden, we published a paper in Gels on a biomimetic wound healing model using cocultures of primary human fibroblasts and macrophages in 3D collagen networks functionalized with sulfated glycosaminoglycans. We show that the network functionalization and the degree of sulfation of GAGs influences the paracrine cell-cell signaling in the in vitro model.
Something’s happening, Germany! Who will ensure less waste?
For the ZDF production “plan b” 2 employees went on the in search of the garbage visionaries. Their goal: To live more resource-consciously and create less waste. For this purpose visited the research laboratory of Dr. Christian Sonnendecker.
You can watch the entire report here: ZDF contribution
Evaluating scaling of capillary photo-biofilm reactors for high cell density cultivation of mixed trophies artificial microbial consortia
Capillary biofilm reactors (CBRs) are attractive for growing photoautotrophic bacteria as they allow high cell-density cultivation. Here, we evaluated the CBR system’s suitability to grow an artificial consortium composed of Synechocystis sp. PCC 6803 and Pseudomonas sp. VBL120. The impact of reactor material, flow rate, pH, O2, and medium composition on biomass development and long-term biofilm stability at different reactor scales was studied. Silicone was superior over other materials like glass or PVC due to its excellent O2 permeability.
Access the full article here:
https://onlinelibrary.wiley.com/doi/10.1002/elsc.202300014
Entropic repulsion of cholesterol-containing layers counteracts bioadhesion
Based on a cooperation with colleagues from Dresden, we newly published a paper in Nature on a new mechanism counteracting bioadhesion by entropic repulsion of orientational fluctuations in cholesterol multilayers. Our results not only reveal this new mechanism of entropic repulsion to be relevant in the surface properties of Collembola, but, also shows the high specificity of the cholesterol chemistry.
You can access the article here:
https://www.nature.com/articles/s41586-023-06033-4
Integrated electrosynthesis and biosynthesis for the production of adipic acid from lignin-derived phenols
To read the article by Dr. Rohan Karande, Alexander Franz, Prof. Dr. Falk Harnisch and Micjel Chávez Morejón click here: https://pubs.rsc.org/en/content/articlelanding/2023/gc/d3gc01105d
Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles
Click here for the article by Prof. Dr. Tilo Pompe, Veronika Riedl, Matthias Portius, Lara Heiser, Philipp Riedl, Torsten Jakob, Rosa Gehring and Thorsten Berg:
https://research.uni-leipzig.de/bact/wp-content/uploads/2023/05/2023-JMatChemB-SCP-SMX.pdf
Read more: Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticlesStructural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains
Click here for the article by Dr. Henri Franquelim: https://www.cell.com/biophysj/fulltext/S0006-3495(23)00135-2
Biomaterial approaches for engineering and analyzing structure and metabolic states of microbial consortia within biofilms
Click here for the article by Prof. Dr. Tilo Pompe and Matthias Portius and Christian Dannenberg:
https://www.sciencedirect.com/science/article/pii/S0958166923000265?dgcid=author