Cell Techniques and Applied Stem Cell Biology

 



 

Equipment

Fluorescence microscope with camera
Zeiss "Axiovert 200"

Objectives: 2,5x, 5x, 10x, 20x, 40x, 63x

Filters: DAPI, FITC special, Cy 3

High resolution color camera "AxioCam HRc"

Software "AxioVision", multichannel fluorescences
   



 

Fluoreszenz reader
"Tecan Safire" with software "Magellan"

High-End multi-functional double-monochromator

Photometer for fluorescence intensity, time resoluted  fluorescence and absorption

FI metering capacity: 230-850 nm

TRF metering capacity: 230-850 nm

Absorption measurement range: 230-1.000 nm

Fluorescence wavelength -scanning capability

shaker

temperature controlled up to 42°C
   



 

Chip-Device
"Affymetrix 428 Array Scanner" and
"Affymetrix 427 Array Spotting-System"

   



 

Crosslinker
UVP "CL-1000 UV-Crosslinker", 254 nm


   



 

Photometer
"LIBRA S22" UV/VIS Spectralphotometer

Wavelenght 190-1.100 nm, Bandwidth < 3 nm

Absorption metering capacity -3.000 up to +3.000 A
   



 

Kryostat
Leica "CM 3050S" with motor and object cooling function

   



 

Mikrotom
Leica "RM 2165" Universal-high- performance mikrotom

   



 

Cytospin
Shandon "Cytospin 4" cyto-centrifuge

   



 

Fast Prep
Qbiogene "Fast Prep Instrument"

   



 

Geldocumentation-system
Mediasystem "FlexiLine 4040", Darkchamber "DH-1",
UV-Transilluminator "UXT-20M-15R"

   



 

MACS
Miltenyi Biotec Ltd. "VarioMacs"

   



 

Freezing-Unit
Thermo Forma computer controlled freezing device, Top & Front access

   



 

Rotation-Vacuum-concentrator
Thermo Electron "SpeedVac Plus Vacuumconcentrator"

   



 

Glucose/Lactat- Analyser
Yellow Springs "YSI 7100 MBS"

Sample volume 10-50 µl

measurement time 1. Module 65 sec., 2. Module 100 sec.

Metering capacity glucose: 0-18,0 g/l, Lactat. 0-2,70 g/l

Auto-sampler for 30 Probes
   



 

Encapsulator
Innotech "Encapsulator IE-50R"

   



 

FACS Device
BD "FACSCalibur"

Green-, orange- and 2 red fluorescenzes

Cellsortingmodul

Cell Concentrator Module
   
 
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