- Inverted confocal laser-scanning microscope Carl
Zeiss LSM510-META
with UV and Vis Argon lasers, HeNe laser units
(351, 364, 457,
488, 514, 543, and 574 nm). Multi-user device
- Prismless TIRF (total internal reflection
fluorescence) microscope
with AOTF-controlled Argon laser (457, 488, and 514 nm). Cooled CCD
camera, DualView
emission beamsplitter for TIR-FRET imaging, and patch-clamp
electrophysiology (HEKA / L&N)
- A TIR-FRET, TIR-FLIM, TIR-FRAP
microscope. Total internal reflection fluorescence excitation (TIRF) is
applied in the prismless configuration (Zeiss alpha-Plan Fluar
100x/1.45) in a custom-built inverted microscope. CW-laser:
AOTF-controlled multiline Argon laser (457, 488, or 514 nm); pulsed
lasers: two diode laser heads with 440 and 470 nm. Probe manipulation:
piezo-controlled
XYZ nanopositioning system (PIMars P-563.3CD). Sensors:
-70°C-cooled, back-illuminated 16-bit
EMCCD camera (Andor BV-887) and two PMT detectors and
time-correlated single photon counting (TCSPC; Pico-Quant) for
fluorescence lifetime/FRET and anistotropy decay analyses
- Two digital fluorescence video imaging and
fluorescence resonance energy
transfer (FRET) imaging setups (Agilent-TILL-Photonics / PCO) attached
to Axiovert 100 inverted microscopes
- Digital fluorescence video
imaging (Agilent-TILL-Photonics /
PCO) with inverted Olympus microscope
- Various software tools for on-the-fly
spectral fingerprinting, indicator calibration, FRET-analysis, FRAP and
FLIM imaging
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