Project B06 – Dynamic modulation of adhesion GPCR function through complex formation

The activity profile and signaling capacity of adhesion GPCRs (aGPCR) may be modulated through crosstalk between different cell surface molecules and may be triggered and/or modulated by mechanical input. We will identify ligand-receptor complexes of aGPCRs through in vitro and in vivo orthogonal labeling and photo-crosslinking as well as intermolecular FRET approaches, respectively. In a further tier of experiments, we will investigate if putative interactors affect aGPCR signaling in a mechano-dependent fashion, test if mechanical stimuli serve as a switch for aGPCR dimerization/oligomerization and investigate how the viscoelastic properties of these interactions correlate with aGPCR activity.

Contact

Resources

in vitro and in vivo orthogonal labeling and photo-crosslinking as well as intermolecular FRET approaches

Publications

2023

Scholz N, Dahse AK, Kemkemer M, Bormann A, Auger GM, Vieira Contreras F, Ernst LF, Staake H, Körner MB, Buhlan M, Meyer-Mölck A, Chung YK, Blanco-Redondo B, Klose F, Jarboui MA, Ljaschenko D, Bigl M, Langenhan T. Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation. Nature. 2023; 615:945-953. doi: 10.1038/s41586-023-05802-5 .

2022

Götze KJ, Mrestani A, Beckmann P, Krohn K, Le Duc D, Velluva A, Böhme MA, Heckmann M, Abou Jamra R, Lemke JR, Bläker H, Scholz N, Ljaschenko D, Langenhan T. Improving one-step scarless genome editing in Drosophila melanogaster by combining ovoD co-CRISPR selection with sgRNA target site masking. Biol Methods Protoc. 2022 Jan 12;7(1):bpac003. doi: 10.1093/biomethods/bpac003 . PMID: 35087953 ; PMCID: PMC8789338.

2021

Beliu G, Altrichter S, Guixà-González R, Hemberger M, Brauer I, Dahse AK, Scholz N, Wieduwild R, Kuhlemann A, Batebi H, Seufert F, Pérez-Hernández G, Hildebrand PW, Sauer M, Langenhan T. Tethered agonist exposure in intact adhesion/class B2 GPCRs through intrinsic structural flexibility of the GAIN domain. Mol Cell. 2021 Mar 4;81(5):905-921.e5. doi: 10.1016/j.molcel.2020.12.042. Epub 2021 Jan 25.

Smolič T, Tavčar P, Horvat A, Černe U, Halužan Vasle A, Tratnjek L, Kreft ME, Scholz N, Matis M, Petan T, Zorec R, Vardjan N. Astrocytes in stress accumulate lipid droplets. Glia. 2021 Feb 20. doi: 10.1002/glia.23978. Epub ahead of print. PMID: 33609060. https://doi.org/10.1002/glia.23978.

2020

Dannhäuser S, Lux TJ, Hu C, Selcho M, Chen JT, Ehmann N, Sachidanandan D, Stopp S, Pauls D, Pawlak M, Langenhan T, Soba P, Rittner HL, Kittel RJ. Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination. Elife. 2020 Sep 30;9:e56738. doi: 10.7554/eLife.56738 PMID: 32996461; PMCID: PMC7546736.

Mathiasen S, Palmisano T, Perry NA, Stoveken HM, Vizurraga A, McEwen DP, Okashah N, Langenhan T, Inoue A, Lambert NA, Tall GG, Javitch JA. G12/13 is activated by acute tethered agonist exposure in the adhesion GPCR ADGRL3. Nat Chem Biol. 2020 Aug 10. doi: 10.1038/s41589-020-0617-7. Epub ahead of print. Erratum in: Nat Chem Biol. 2020 Aug 17;: PMID: 32778842. [publisher correction: doi: 10.1038/s41589-020-0649-z]

2019 and earlier

Scholz N^*, Ehmann N^*, Sachidanandan D, Imig C, Cooper BH, Jahn O, Reim K, Brose N, Meyer J, Lamberty M, Altrichter S, Bormann A, Hallermann S, Pauli M, Heckmann M, Stigloher C, Langenhan T^#, Kittel RJ^#. Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix. J Cell Biol. 2019; 218:1011-26.

Scholz N, Langenhan T, Schöneberg T. Revisiting the classification of adhesion GPCRs. Ann N Y Acad Sci. 2019; 1456: 80-95.

Blanco Redondo B, Langenhan T#. Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP Sites. G3 (Bethesda). 2018; 8:3109–3118.

Scholz N. Cancer Cell Mechanics: Adhesion G Protein-coupled Receptors in Action? Front Oncol. 2018; 8:59.

Scholz N*, Guan C*, Nieberler M*, Grotemeyer A*, Maiellaro I, Gao S, Beck S, Pawlak M, Sauer M, Asan E, Rothemund S, Winkler J, Prömel S, Nagel G, Langenhan T^#, Kittel RJ^#. Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons. 2017; 6:e28360.

Langenhan T^#, Piao X^#, Monk KR^#. Adhesion G protein-coupled receptors in nervous system development and disease. Nat Rev Neurosci. 2016; 17:550-61.

Scholz N^#, Monk KR, Kittel RJ, Langenhan T^#. Adhesion GPCRs as a Putative Class of Metabotropic Mechanosensors. Handb Exp Pharmacol. 2016; 234:221-47.

Scholz N*, Gehring J*, Guan C*, Ljaschenko D, Fischer R, Lakshmanan V, Kittel RJ^#, Langenhan T^#. The Adhesion GPCR Latrophilin/CIRL Shapes Mechanosensation. Cell Rep. 2015; 11:866-74.

Langenhan T*, Aust G*, Hamann J*. Sticky Signaling-Adhesion Class G Protein-Coupled Receptors Take the Stage. Sci Signal. 2013; 6:re3.

Prömel S^#, Waller-Evans H, Dixon J, Zahn D, Colledge WH, Doran J, Carlton MBL, Grosse J, Schöneberg T, Russ AP, Langenhan T^#. Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse. Dev Dyn. 2012b; 241:1591-602.

Prömel S, Frickenhaus M, Hughes S, Mestek L, Staunton D, Woollard A, Vakonakis I, Schöneberg T, Schnabel R, Russ AP, Langenhan T. The GPS motif is a molecular switch for bimodal activities of adhesion class G protein-coupled receptors. Cell Rep. 2012; 2:321-31.

Langenhan T, Prömel S, Mestek L, Esmaeili B, Waller-Evans H, Hennig C, Kohara Y, Avery L, Vakonakis I, Schnabel R, Russ AP. Latrophilin signaling links anterior-posterior tissue polarity and oriented cell divisions in the C. elegans embryo. Dev Cell. 2009; 17:494-504.

Project B06 – Dynamic modulation of adhesion GPCR function through complex formation

Contact

Dr. Nicole Scholz (Project Leader)

Prof. Dr. D.Phil. (Oxon) Tobias Langenhan (Project Leader)

Anne-Kristin Dahse (PhD Student and Vice-Representatives of the PhD students of the SFB1423)

Anne Bormann (PhD Student)

Resources

Publications