The scientific module “Expression and Preparation of GPCRs” will take place October 5-7, 2020.
Topics include:
- protein production of extracellular domains in HEK293 cells and protein quality control
- protein glycosylation and its control in HEK293 cell expression
- E.coli expression
- cell free expression
- functional reconstitution in lipid membranes
- Sf9/Hi5 expression of GPCRs and G-proteins
- solubilization and purification
- sample preparation for X-ray crystallography or Cryo-EM
- and more
Participating PIs:
- Prof. Dr. Norbert Sträter
- Dr. Peter Schmidt
- Dr. Patrick Scheerer
A detailed program will follow soon.
Who:
PhD students of the CRC
(there will be a waiting list for external PhD students)
Where:
Day 1 BBZ, Leipzig
Day 2 Härtelstraße, Leipzig
Day 3 online