Scientific Module – Expression and Preparation of GPCRs

Scientific Module – Expression and Preparation of GPCRs
  • Beitrags-Autor:
  • Beitrag veröffentlicht:9. September 2020

The scientific module “Expression and Preparation of GPCRs” will take place October 5-7, 2020.

Topics include:

  • protein production of extracellular domains in HEK293 cells and protein quality control
  • protein glycosylation and its control in HEK293 cell expression
  • E.coli expression
  • cell free expression
  • functional reconstitution in lipid membranes
  • Sf9/Hi5 expression of GPCRs and G-proteins
  • solubilization and purification
  • sample preparation for X-ray crystallography or Cryo-EM
  • and more

Participating PIs:

  • Prof. Dr. Norbert Sträter
  • Dr. Peter Schmidt
  • Dr. Patrick Scheerer

A detailed program will follow soon.

Who:
PhD students of the CRC
(there will be a waiting list for external PhD students)

Where:
Day 1 BBZ, Leipzig
Day 2 Härtelstraße, Leipzig
Day 3 online