We cordially invite you to a talk about
“Cryo-Electron Microscopy of Cellular Metabolism“
by Jun-Prof. Dr. Panagiotis Kastritis
Martin-Luther University of Halle-Wittenberg, Halle (Saale), Germany
Tuesday, December 15th, at 4 p.m. Leipzig.
Cryo-electron microscopy (cryo-EM) is used to characterize the structure of radiation-sensitive specimen, such as biological macromolecules. In cryo-EM, the sample is rapidly frozen in liquid ethane and kept at liquid nitrogen temperatures. It is then transferred to a cryo-microscope stage to be imaged while such low temperatures allow its structural preservation. By applying low-dose imaging protocols, efficient recording of high-resolution micrographs on advanced detectors is achieved and, finally, after image processing, high-resolution three-dimensional (3D) reconstructions are attainable. My laboratory is interested to derive 3D structures and interactions of biological macromolecules by cryo-EM and integrate EM data with biochemical, biophysical and biocomputational methods. In my talk, I will introduce cryo-EM and its importance in the structural research of biological macromolecules. Then, I will present cryo-EM applications on systems of interest, ranging from simpler polymer samples to complex biological macromolecules. I will conclude by highlighting the need to probe the structural organization of native cell extracts. Native cell extracts provide opportunities to uncover biomolecular states of metabolic complexes previously unknown.
Join via Zoom:
Meeting-ID: 982 3133 7927