Short Report by Hamzah Khawaja: Exploring the impact of multiple protease treatments on PAR-4 mediated G protein signaling and NLRP3 Inflammasome activation (March-June)
I knew my first trip to the US would be memorable, but I never imagined it would be this amazing. From the bustling streets of downtown Nashville to the serene lakeside at Hamilton Creek, the city is a sight to behold. Even more impressive was the cutting-edge research at Vanderbilt University (VU). As a junior scientist, it was thrilling to work with experts across various fields and deepen my understanding of pharmacodynamics in molecular biology.
My PhD research focuses on the regulation of the NLRP3 inflammasome by PAR4. To address this, I aimed to learn techniques for proximal G protein signaling at the Hamm Lab at VU. Under the guidance of Prof. Heidi Hamm and her team, I optimized and standardized the TRUPATH and Nanobit BRET assays for PAR4 signaling and conducted flow cytometry experiments to observe the effects of various proteases on platelet activation and inflammasome signaling via PAR4 activation.
During my stay, I was immersed in the vibrant atmosphere of Music City, exploring landmarks like the Grand Ole Opry and the Country Music Hall of Fame. In contrast, my visit to Columbus, Ohio, offered a diverse cultural experience with its thriving arts scene and innovative culinary landscape, particularly in the Short North Arts District and North Market. This trip was an unforgettable blend of professional growth and cultural exploration, leaving me inspired and eager to apply what I’ve learned to my ongoing research.
