Call 2023: Offering research internship for Students on Bachelor- or Master level (Deadline is August 15, 2022)

Call 2023: Offering research internship for Students on Bachelor- or Master level (Deadline is August 15, 2022)

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three‐four undergraduate students from each university to conduct a 10 week summer internship at the respective partner university. Travel costs and a weekly stipend are provided. They target rising junior and rising senior students at Vanderbilt University. To select the recipients of the stipend, we collect research project descriptions from around twenty core laboratories that support the collaboration between Vanderbilt and Leipzig Universities. Descriptions are provided by the graduate student or post‐doctoral mentor who will advise the undergraduate student. It is encouraged that the undergraduate student conducts research on the

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01-05.08.2022:  Scientific Module “ALPHAFOLD, ROSETTA, AND AMBER: Principles and Applications of Computational Structural Biology”

01-05.08.2022:  Scientific Module “ALPHAFOLD, ROSETTA, AND AMBER: Principles and Applications of Computational Structural Biology”

We cordially invite you to a one week block online seminar “ALPHAFOLD, ROSETTA, AND AMBER: Principles and Applications of Computational Structural Biology” by: Dr. Georg Künze, Dr. Clara T. Schoeder, Prof. Dr. Jens MeilerLeipzig University, Medical Faculty Dr. Jarrod Smith, Dr. Rocco Moretti, Dr. Benjamin P. Brown, Dr. Alican Gulsevin, Dr. Cristina E. MartinaVanderbilt University DATE01 – 05. August 2022, EST The class will meet virtually on 5 days in the week from August 1st to August 5th always 3 pm-6 pm . The laboratory portion will proceed on the same dates 7 pm-10 pm. Additionally, a short introduction to the Linux command line will be offered on the first day. This is a one credit course. COURSE DESCRIPTION Proteins

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Virtual and In-Vitro Search of New Ligands for Adhesion G Protein-Coupled Receptor (Short Report by Richard Schreiter; Jan. – April 2022)

Virtual and In-Vitro Search of New Ligands for Adhesion G Protein-Coupled Receptor (Short Report by Richard Schreiter; Jan. – April 2022)

I took part in the Max Kade Foundation exchange program as a part of my 6 months long diploma, an additional and optional degree when studying pharmacy. Prior to my diploma and my stay in Nashville, research experiences were barely existent, as the study program of pharmacy does not notably focus on that. However, before deciding to pursue a diploma in this field, I made two internships of two weeks each that gave me a rudimentary insight into the wet lab and computational work. Also, first contact with the research field of adhesion coupled G protein receptors (AGPCRs) occured during that time because one of my projects covered the determination of

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Expression and purification of arrestin-3 for structural studies (Short Report by Emelyne Pacull; Feb. – April 2022)

Expression and purification of arrestin-3 for structural studies (Short Report by Emelyne Pacull; Feb. – April 2022)

My PhD research at Leipzig University focus on the Growth Hormone Secretagogue Receptor (GHSR), a receptor in our cell membranes involved in food intake and part of the G-protein coupled receptors (GPCRs) family, key targets of pharmacological drugs. More specifically, I am investigating the conformational changes of this receptor in the presence of its ligand ghrelin or other extra and intracellular partners, using solid state NMR. After having to cancel my exchange for the summer 2020 due to the coronavirus pandemic, I finally had the awesome opportunity to work in the lab of Prof. Dr. Vsevolod V. Gurevich from February to April 2022. The main goal of this exchange was to

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Bridging Drug Discovery Pipelines for Challenging Protein Targets in the Institute for Drug Discovery and MeilerLab Vanderbilt  (Short Report by Fabian Liessmann; Jan. – March 2022)

Bridging Drug Discovery Pipelines for Challenging Protein Targets in the Institute for Drug Discovery and MeilerLab Vanderbilt (Short Report by Fabian Liessmann; Jan. – March 2022)

As the chemical space is incredibly large with up to estimated 1060 molecules defined as small-molecule ligand, sophisticated and benchmarked methods and pipelines for screening this huge space in a fast and reliable manner are needed. In recent year, virtual ultra-large library screening has witnessed a rapid development with increasing computational power, but also growing available commercial make-on-demand libraries, like the REadily AccessibLe (REAL) libraryof Enamine Ltd. with currently 21 billion chemicals. Pipelines for screening huge databases are available but rely heavily on screening all the compounds. In the Institute for Drug Discovery in Leipzig an in-silico ultra-large library screening pipeline using flexible models of GPCRs was developed, tested, and established.

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Apply for  Max Kade Scholarship 2022

Apply for Max Kade Scholarship 2022

Unique Opportunities in Gaining Research Experience in Germany and USA The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three undergraduate students from each university to conduct a 10 week summer internship at the respective partner university in 2019 (May – December). The Max Kade Foundation Scholarship Program is open for highly motivated and excellent foreign applicants with a BSc, MSc or equivalent degrees (obtained during the past year). Internship offer in Leipzig for Undergraduate Students from Vanderbilt University  Evolutionary Guided Design of Novel PET-Degrading Enzymes to increase Thermostability Dr. Georg Künze (Institute for Drug Development) Analysis of Ternary

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18.01.2022: Virtual seminar series “AlphaFold at Vanderbilt.”

18.01.2022: Virtual seminar series “AlphaFold at Vanderbilt.”

The Vanderbilt Center for Structural Biology, the Program in Personalized Structural Biology, and the Program in the Molecular Basis of Genetic Diseases will be holding a series of seminars to introduce AlphaFold.  These talks will be held on Tuesday January 18, 2022, from 4:00 p.m. CEST (Leipzig time) via Zoom. SpeakersJens Meiler, PhD (Chemistry)Hassane S. Mchaourab, PhD (Molecular Physiology & Biophysics)David Rinker, PhD (Chemistry)Jarrod Smith, PhD (Biochemistry)Rich Stein, PhD (Molecular Physiology & Biophysics)Taylor Jones (Chemical and Physical Biology Program)Diego Del Alamo (Chemical and Physical Biology Program) Topics+ Ramifications and impact of AlphaFold on biomedical sciences+ AlphaFold and the diagnosis of genetic disorders+ Applications of AlphaFold to personalized medicine+ AlphaFold and AlphaFold-Multimer+

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Call 2021: Offering research internship for Students on Bachelor- or Master level

Call 2021: Offering research internship for Students on Bachelor- or Master level

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three‐four undergraduate students from each university to conduct a 10 week summer internship at the respective partner university. Travel costs and a weekly stipend are provided. They target rising junior and rising senior students at Vanderbilt University. To select the recipients of the stipend, we collect research project descriptions from around twenty core laboratories that support the collaboration between Vanderbilt and Leipzig Universities. Descriptions are provided by the graduate student or post‐doctoral mentor who will advise the undergraduate student. It is encouraged that the undergraduate student conducts research on the

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16-20.08.2021: Module “Exploring Biomolecules in 3D: Principles and Applications of Computational Structural Biology”

16-20.08.2021: Module “Exploring Biomolecules in 3D: Principles and Applications of Computational Structural Biology”

We cordially invite you to a one week block online seminar “Exploring Biomolecules in 3D: Principles and Applications of Computational Structural Biology” by: Prof. Dr. Jens Meiler(Leipzig University, Coordinator)Dr. Georg Kuenze(Leipzig University)Prof. Dr. Jarrod Smith(Vanderbilt University / USA)Prof. Dr. Rocco Moretti(Vanderbilt University / USA) DATE16 – 20. August 2021, EST (13. August 2021 technical setup) The class will meet virtually on 5 days in the week from August 16th to August 20th always 8:00 a.m. -11:00 a.m. CDT / 3:00 p.m. – 6:00 p.m. CEST. The laboratory portion will proceed on the same dates 12:00 p.m. – 3:00 p.m. CDT / 7:00 p.m. – 10:00 p.m. CEST. The laboratory portion on August 13th is indented to sort out

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25.03.2021 virtual talk “Structure of the rhodopsin–rhodopsin kinase complex defines the rules of engagement between G protein-coupled receptors (GPCRs) and GPCR kinases” (Dr. Qiuyan Chen (Prof. John Tesmer lab / Purdue University / USA)

25.03.2021 virtual talk “Structure of the rhodopsin–rhodopsin kinase complex defines the rules of engagement between G protein-coupled receptors (GPCRs) and GPCR kinases” (Dr. Qiuyan Chen (Prof. John Tesmer lab / Purdue University / USA)

We would like to invite you to our next VULU Lecture given by Dr. Qiuyan Chen (Prof. John Tesmer lab / Purdue University / USA) Title:Structure of the rhodopsin–rhodopsin kinase complex defines the rules of engagement between G protein-coupled receptors (GPCRs) and GPCR kinases

07-18.12.2020: Module “Solution NMR integrated with Computational Modeling to study Membrane Proteins”

07-18.12.2020: Module “Solution NMR integrated with Computational Modeling to study Membrane Proteins”

We cordially invite you to a two week block online seminar “Solution NMR integrated with Computational Modeling to study Membrane Proteins” by: Prof. Dr. Jens Meiler(Leipzig University, Vanderbilt University / USA, Organizer)Prof. Dr. Chuck Sanders(Vanderbilt University / USA)Dr. Georg Künze(Leipzig University, Vanderbilt University / USA)Dr. Davide Sala(Leipzig University) DATE07 – 18. December 2020, 3 – 5 p.m. CEST (15:00 – 17:00 Uhr Leipzig). AIMSTeach theoretical and practical aspects of integrating solution NMR with computational modelling to study membrane protein computational structure, dynamics, and function.BASICSBiochemistry, amino acid structure, peptide bond, secondary structure, tertiary structure, biological membrane, protein sensors, protein transporters, protein channelsCOURSE DESCRIPTION The Module includes theoretical and practical aspects of protein sequence alignments, secondary structure

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29.11.2020: Virtual Talk “Small-molecule inhibitors of cancer-related protein-protein interactions” (Prof. Thorsten Berg, Faculty of Chemistry and Mineralogy at Leipzig University)

29.11.2020: Virtual Talk “Small-molecule inhibitors of cancer-related protein-protein interactions” (Prof. Thorsten Berg, Faculty of Chemistry and Mineralogy at Leipzig University)

We would like to invite you to our next VULU Lecture given by Prof. Dr. Thorsten Berg, Faculty of Chemistry and Mineralogy at Leipzig University “Small-molecule inhibitors of cancer-related protein-protein interactions“ Protein-protein interactions form the basis of most biological processes, but represent very challenging targets for small organic molecules. The focus of our current research is to develop and apply innovative methods for the efficient design of small organic modulators of protein-protein interactions. These methods are applied for the synthesis and functional characterization of highly potent modulators of pre-selected protein-protein interactions. The presentation will give an overview on our recent work in the following areas: 1) development of selective inhibitors of

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07.-21.08.2020: Virtual Course “Computational methods to study protein structure and dynamics” (Hildebrand, Meiler, Smith, Moretti)

07.-21.08.2020: Virtual Course “Computational methods to study protein structure and dynamics” (Hildebrand, Meiler, Smith, Moretti)

Syllabus – CPBP 8330 – Special Topics in Protein BiochemistryCOMPUTATIONAL METHODS TO STUDY PROTEIN STRUCTURE AND DYNAMICS Course Description:Biomolecules undergo constant motions and fluctuations in their native environment. Many of these motions are directly related to function: Channels have to open or close, transporters change their form to conduct molecules and receptors undergo conformational changes to transmit signals through membranes. Despite that obvious dynamics, biomolecules are still commonly considered as rigid entities that exist in one or only few states. This limitation largely prevents understanding of molecular events that are guided or even ruled by intrinsic flexibility such as ligand binding or protein-protein recognition. Molecular dynamics (MD) simulation is a well-established

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Scientists find human antibodies against COVID-19

Scientists find human antibodies against COVID-19

[article written by Peggy Darius Leipzig University] The COVID 19 pandemic poses a major threat to global health and currently there is little medical response. In addition, there is a lack of a profound understanding of the molecular mechanisms of the body’s immune system. An international team has now succeeded in scientifically describing highly active neutralizing human antibodies against the corona virus SARS-CoV-2. Humboldt Professor Jens Meiler of the University of Leipzig contributed to the study, which has now been published in the scientific journal “Nature”. Highly active neutralizing human antibodies against the coronavirus SARS-CoV-2 can be used for the development of vaccines and can also be used as therapy in

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Old home and new challenge

Old home and new challenge

[article only in german language, article written by Dr. Katarina Werneburg Leipzig University / picture: Christian Hüller / Universität Leipzig, SUK] Alte Heimat und neue Herausforderung Was sind die Forschungsschwerpunkte unserer Professoren? Was ist ihnen in der Lehre wichtig, und haben unsere Experten eigentlich auch Hobbys oder ein Lebensmotto? Im LUMAG stellt sich auch heute wieder einer unserer neuberufenen Professoren vor. Diesmal ist es Jens Meiler vom neu gegründeten Institut für Wirkstoffentwicklung. Sein Fachgebiet ist die computergestützte Wirkstoffforschung. Herr Meiler, woher kommen Sie und was haben Sie studiert? Ich bin in Leipzig geboren und aufgewachsen. Nach meinem Abitur am Wilhelm-Ostwald Gymnasium studierte ich Chemie an der Universität Leipzig. Was waren Ihre

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31.01.2020 virtual talk “Computational investigation of (metallo) proteins structure and dynamics” (Dr. Davide Sala, Fondazione Sacconi, Milan, Italy)

31.01.2020 virtual talk “Computational investigation of (metallo) proteins structure and dynamics” (Dr. Davide Sala, Fondazione Sacconi, Milan, Italy)

We would like to invite you to our next VULU Lecture given by Dr. Davide SalaFondazione Sacconi, Milan, Italy Computational investigation of (metallo) proteins structure and dynamics Abstract Proteins that require one or more metal ions to perform their biochemical function are called metalloproteins. To date, metalloproteins are estimated to be one-third of all proteins in most of the organisms and are involved in many fundamental biochemical processes. In this regard, the large diffusion of powerful and cost-effective GPGPU units has allowed the computational exploration of a number of biological processes using techniques based on molecular dynamics (MD). Thus, computational methods as MD can be exploited to investigate metalloproteins structural properties

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Short Report by Sarina Rudolf:  Incorporating crosslinking data into Rosetta to dock NPY and Y5R . (Oct. – Dec. 2019)

Short Report by Sarina Rudolf: Incorporating crosslinking data into Rosetta to dock NPY and Y5R . (Oct. – Dec. 2019)

In the recent years, we pursued the goal to map the binding site of the ligand NPY at human neuropeptide Y5 receptor (Y5R). Therefore, we applied an approach called genetic code expansion to incorporate a photoreactive amino acid at a desired site at the extracellular part of the receptor. The obtained photocrosslinking-data revealed the proximity of several receptor positions to NPY in the ligand-receptor complex. Hence, giving us deeper insights into the receptor-binding pocket. For the visualization of my experimental data in a comparative model, I got the opportunity to perform the computational modeling in the laboratories of Jens Meiler at the Vanderbilt University in Nashville, Tennessee. In the course of

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Short Report by Victoria Most:  The sun in Nashville and a light-sensitive protein. (Aug.-Nov. 2019)

Short Report by Victoria Most: The sun in Nashville and a light-sensitive protein. (Aug.-Nov. 2019)

As a Master candidate of the Biochemistry program at Leipzig University I focus on analytics and structural biology. My studies mainly concentrated on performing experiments in the wet lab and I had only little knowledge of Rosetta and scientific programming.               During my stay I worked on the computational modelling of G protein-coupled receptors (GPCRs) using the Rosetta homology modelling protocol. GPCRs are membrane proteins, which are crucial for translating extracellular signals into an intracellular response. It has been shown that these proteins are prone to misfolding during insertion into the membrane. During my research I tried to model a proposed misfolded state of the light-sensitive GPCR Rhodopsin[1] and managed to

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University Success for Three Collaborative Research Centres

University Success for Three Collaborative Research Centres

[Press Release 25/11/2019, Leipzig University 234/2019 by Carsten Heckmann / Photo: Leipzig University, Swen Reichhold] Further boost for top-level research at Leipzig University: New Collaborative Research Centre gets go-ahead and two more are extended. The University’s new Collaborative Research Centre (CRC), “Structural Dynamics of GPCR Activation and Signal Transduction”, will focus on how cells communicate via important receptors. Its spokesperson will be Professor Annette G. Beck-Sickinger. Applications for a second funding period were also successful for the projects “Arctic Climate Change (AC)³” (spokesperson: Professor Manfred Wendisch, Leipzig Institute for Meteorology) and “Processes of Spatialization under the Global Condition” (spokesperson: Professor Matthias Middell, head of Leipzig University’s Centre for Area Studies). Brief

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Dr. Torben Schiffner: HIV vaccine development using rational protein design

Dr. Torben Schiffner: HIV vaccine development using rational protein design

December 19, 2019; 12:30: Talk at the at Fraunhofer Institute for Cell Therapy and Immunology – IZI Abstract: Despite decades of intensive research, a vaccine capable of protecting against HIV-1 infection remains elusive. The immuno-evasion strategies employed by the HIV-1 envelope glycoprotein have been a significant challenge to progress in the field. However,  antibodies capable of neutralizing most circulating HIV-1 strains have been isolated from infected patients, thus providing templates for vaccine development. In this seminar, approaches will be discussed that utilize rational protein design to develop immunogens which specifically activate B cells from broadly neutralizing antibody lineages, which may lead to the development of a prophylactic HIV vaccine. Contact: Prof.

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Call 2020: Offering research internship for Students on Bachelor- or Master level

Call 2020: Offering research internship for Students on Bachelor- or Master level

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three‐four undergraduate students from each university to conduct a 10 week summer internship at the respective partner university. Travel costs and a weekly stipend are provided. They target rising junior and rising senior students at Vanderbilt University. To select the recipients of the stipend, we collect research project descriptions from around twenty core laboratories that support the collaboration between Vanderbilt and Leipzig Universities. Descriptions are provided by the graduate student or post‐doctoral mentor who will advise the undergraduate student. It is encouraged that the undergraduate student conducts research on the

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Prof. Tony Capra: Diagnosing the Legacy of Neanderthal Interbreeding on Modern Humans

Prof. Tony Capra: Diagnosing the Legacy of Neanderthal Interbreeding on Modern Humans

December 12, 2019; 14:00: Talk at the Department of Evolutionary Genetics Location:Seminar Area Genetics Abstract: [will be annouced soon] Contact: Max-Planck-Institut für evolutionäre Anthropologie Janet Kelso (mail: ) Website: https://www.eva.mpg.de/genetics/index.html

Lecture on “Modeling protein mutations with Rosetta” at Leipzig University

Lecture on “Modeling protein mutations with Rosetta” at Leipzig University

December 05-19, 2019. This course teach theoretical and practical aspects of computational biology including the integration of Human Genetics with Molecular Modeling. It focus on aspects of protein sequence alignments, secondary structure prediction, comparative modeling, protein-protein and protein-ligand docking, structure-based drug design, virtual screening, the relationship of protein sequence, structure, variation, and disease. This course includes a practical section and is intended for PhDs, PostDocs and Students. 05.-06.12.2019, 9 AM – 04 PM Module 1: STRUCTURE BASED MOLECULAR MODELINGProf. Jens Meiler (Vanderbilt University/USA) *Introduction to Structural Biology *Protein Structure Prediction with Rosetta *Protein Structure Determination from Limited Experimental Data * Protein Engineering and Design 09.-12.12.2019, 09 AM – 1 PM Module

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Lecture on “New approaches for practical and time efficient cloning, construct design and the strategic selection of protein-protein interaction techniques” at Vanderbilt University

Lecture on “New approaches for practical and time efficient cloning, construct design and the strategic selection of protein-protein interaction techniques” at Vanderbilt University

September 4-10, 2019: Syllabus – CPBP 8330 – Special Topics in Protein Biochemistry: Dr. Dr. Ines LiebscherLeipzig University, Medical Faculty, Institute of Biochemistry Date 04 – 10 September 2019 VenueDepartments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, Room 5131, Nashville, TN 37232-8725 Program: Research techniques are always changing, they develop and improve and sometimes it is hard to stay fully informed. In this module we will talk about different methods and techniques you want to use to be most efficient in your project. In the first half of the module we will introduce you to the basics and

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Short Report by Johanna Tiemann: Arrestin interaction with SRC family kinases. (Apr. 2019)

Short Report by Johanna Tiemann: Arrestin interaction with SRC family kinases. (Apr. 2019)

Johanna K. S. Tiemann1, Prof. Dr. Vsevolod Gurevich2, Dr. Sandra Berndt2 1. Leipzig University 2. Vanderbilt University The aim of my exchange was to deepen the collaboration between the laboratory of Prof. Peter Hildebrand and Prof. Tina Iverson and Prof. Seva Gurevich and to push the project on the investigation of arrestin interactions with SRC family kinases forward. Arrestin is a key intracellular downstream signaling molecule, mediating G protein-coupled receptors and other proteins such as kinases. Those proteins are involved in tumor progression and metastasis, their study, therefore, of noticeable interest. Especially the interaction of arrestin with kinases, in particular, SRC family kinases is so far barely understood. Through the established

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The Alexander von Humboldt Foundation awarded Prof. Dr. Jens Meiler

The Alexander von Humboldt Foundation awarded Prof. Dr. Jens Meiler

[article written by Dr. Katarina Werneburg Leipzig University / picture: Humboldt-Stiftung/Elbmotion] The Alexander von Humboldt Foundation awarded Prof. Dr. Jens Meiler the highly endowed Alexander von Humboldt Professorship. The 44-year-old is one of the world’s most distinguished researchers in the field of computer-aided drug discovery. The Alexander von Humboldt Professorship is Germany’s most valuable research prize. “I am delighted that the long-standing research partnership between Leipzig University and Vanderbilt University has now culminated in Jens Meiler’s Humboldt Professorship. This will unlock new horizons for Professor Beck-Sickinger’s already highly successful area of research into molecular mechanisms. It will also considerably strengthen our degree program in Pharmacy at the Faculty of Medicine,” commented

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Apply for  Max Kade Scholarship 2019

Apply for Max Kade Scholarship 2019

Unique Opportunities in Gaining Research Experience in Germany and USA The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three undergraduate students from each university to conduct a 10 week summer internship at the respective partner university in 2019 (May – December). The Max Kade Foundation Scholarship Program is open for highly motivated and excellent foreign applicants with a BSc, MSc or equivalent degrees (obtained during the past year). Internship offer in Leipzig for Undergraduate Students from Vanderbilt University  Analysis of Ternary Complexes of G-Protein Coupled Receptors Using Molecular Dynamics Simulations. Prof. Dr. Hildebrand/Dr. Vogel (Institute of Biophysics) Structural

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Short Report by Paul Eisenhuth: Rosetta docking of allosteric modulators to the Y4 receptor. (Sept. – Dec. 2018)

Enhancing pathogenicity prediction from structure and evolution with machine learning Currently I am enrolled in a Computer Science Master program at the University of Leipzig. Since my program offers no practical experience in scientific research I was excited when I got offered to participate in the Max Kade Foundation Fellowship. During my ten weeks stay I started a completely new project with the goal to develop a pathogenicity prediction score on point variants in human genes incorporating structural and evolutionary features. Obviously, the scope of this project was way to big to be fulfilled during ten weeks only, so I aimed to collect some data, calculate features and train a machine

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Short Report by Tobias Fischer: Investigation of Ligand Binding at the Chemokine-Like Receptor 1. (Sept. – Dec. 2018)

Short Report by Tobias Fischer: Investigation of Ligand Binding at the Chemokine-Like Receptor 1. (Sept. – Dec. 2018)

I am a PhD student in Leipzig in the lab of Annette Beck-Sickinger. My research focuses on investigation of ligand-binding at a speci c G protein-coupled receptor, the chemokine-like receptor 1. This receptor is involved in multiple in ammatory diseases and therefore is a promising drug target. While we have the possibility to investigate the pharmacology of this receptor here in Leipzig, the lab of Jens Meiler at Vanderbilt offers computational methods to improve understanding of this receptor on a molecularlevel. Hence, I spent 10 weeks in Vanderbilt, where I had the possibility to learn using the software suite Rosetta, which includes a variety of algorithms for protein structure prediction and is

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Short Report by Anja Wieprecht: Identification and verification of interaction partners of adhesion G protein-coupled receptors. (Aug. – Oct. 2018)

Adhesion G protein-coupled receptors (aGPCRs) are expressed in various cell types, including cells of the immune system and central nervous system. Furthermore, they were reported to be involved in tumorgenesis. Besides their physiological relevance the majority of them have not been functionally characterized in detail. Notably, in contrast to other GPCRs this class of receptors possess an autoproteolytical site within their N-terminus at which they are cleaved. It is known that aGPCRs can be activated by their Stachel-sequence, a short tethered agonist sequence N-terminal of this cleavage site. However, both the binding pocket and the mechanism of its activation remain to be characterized. Using the smallest and noncleaved GPR114 as a

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Lecture on “Modeling Proteins guided by Electron Paramagnetic Resonance and Mass Spectrometry” at Leipzig University

Lecture on “Modeling Proteins guided by Electron Paramagnetic Resonance and Mass Spectrometry” at Leipzig University

December 11-18, 2018; Biochemical Lecture – Special Topics in Biochemistry Lecture on “Modeling Proteins guided by Electron Paramagnetic Resonance and Mass Spectrometry” 11.-14.12.2018, 01 PM – 04 PM Module 1: Molecular Modeling Prof. Jens Meiler (Vanderbilt University/USA) *Introduction to Structural Biology *Protein Structure Prediction with Rosetta *Protein Structure Prediction from EPR DATA 17.-19.12.2018, 09 AM – 4 PM Module 2: Biomolecular electron paramagnetic resonance spectroscopy (EPR) Prof. Eric Hustedt (Vanderbilt University/USA) *Introduction to Site-Directed Spin Labeling (SDSL) and Electron Paramagnetic Resonance (EPR) Spectroscopy *Distance Measurements and Pulsed EPR *Discussion of Significant EPR Papers Module 3: Molecular Dynamics MS-based Modeling Prof. Steffen Lindert (Ohio State University/USA) *Molecular Mechanics *Molecular Dynamics *Protein Structure

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Summer School on “Peptide-Activated GPCRs: Ligand Binding And Signal Transduction” at Vanderbilt University

Summer School on “Peptide-Activated GPCRs: Ligand Binding And Signal Transduction” at Vanderbilt University

October 20-24, 2018; Syllabus – CPBP 8330 – Special Topics in Ligand-Protein Recognition Prof. Dr. Annette G. Beck-Sickinger, Leipzig University, Faculty of Life Sciences, Institute of Biochemistry Date October 20-24, 2018 Venue Departments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, Room 5131, Nashville, TN 37232-8725 The student will become familiar with the synthesis, analysis, chemical modification and biological testing of peptide and protein GPCR ligands. Many ligands of GPCR belong to the family of peptides and proteins. This includes all class B GPCR ligands, e. g. calcitonin, glucagon and the pharmacologically relevant GLP-1, from which modified analogies have

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Researchers of Vanderbilt and Leipzig University discover ligand binding modes at the neuropeptide Y Y1 receptor

Researchers of Vanderbilt and Leipzig University discover ligand binding modes at the neuropeptide Y Y1 receptor

(30/04/2018 by Anett Albrecht): A joint publication of Leipzig and Vanderbilt scientists was published in April 2018 in the prestigious journal “Nature”. The article continues the series of numerous publications that have emerged already from the long-term collaboration between the Leipzig and Vanderbilt University. Abstract: Neuropeptide Y (NPY) receptors belong to the G-protein-coupled receptor superfamily and have important roles in food intake, anxiety and cancer biology 1,2 . The NPY-Y receptor system has emerged as one of the most complex networks with three peptide ligands (NPY, peptide YY and pancreatic polypeptide) binding to four receptors in most mammals, namely the Y1, Y2, Y4 and Y5 receptors, with different affinity and selectivity 3

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Universities extend partnership by another ten years

Universities extend partnership by another ten years

[press release written by Susann Huster/Anett Albrecht, April 10, 2018] The cooperation between the University of Leipzig and Vanderbilt in Nashville (USA) has been contractually sealed for another ten years. A few days ago, the rectors of both universities signed a Memorandum of Understanding. The American Max Kade Foundation had previously approved an application for funding from project leaders Prof. Dr. Annette Beck-Sickinger from the Institute of Biochemistry at the Leipzig University and Prof. Dr. Jens Meiler from Vanderbilt University: The foundation will also support the exchange with 50,000 dollars (40,000 euros) per year between 2017 and 2021. “We hope that the already established excellent relations with Vanderbilt University will continue

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Max Kade Scholarship 2018: Unique Opportunities in Gaining Research Experience in Germany and USA

Max Kade Scholarship 2018: Unique Opportunities in Gaining Research Experience in Germany and USA

The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three undergraduate students from each university to conduct a 10 week summer internship at the respective partner university in 2018 (May – September). The Max Kade Foundation Scholarship Program is open for highly motivated and excellent foreign applicants with a BSc, MSc or equivalent degrees (obtained during the past year). Identification of functional protein-protein-interactions of the ecto- and endodomain of adhesion G protein-coupled receptors (Prof. Dr. Vsevolod Gurevich) Investigation of Ligand Binding at the Chemokine-Like Receptor 1 (Prof. Dr. Jens Meiler) Rosetta docking of allosteric modulators to the Y4 receptor.

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Lecture on “Innovative Computational Methods for Protein Structure Prediction, Drug Discovery, and Therapeutic Design” at Leipzig University

Lecture on “Innovative Computational Methods for Protein Structure Prediction, Drug Discovery, and Therapeutic Design” at Leipzig University

Biochemical Colloquium – Special Topics in Biochemistry: Prof. Dr. Jens Meiler, Center for Structural Biology, Departments of Chemistry, Pharmacology, and Biomedical Informatics, Vanderbilt University, Nashville Tenessee Date October 24, 2017 / 5 PM Venue Leipzig University, ITP – Institute for Theoretical Physics, Lecture Hall, Linnéstraße 5, 04103 Leipzig, Germany. Backround The protein folding problem – i.e. predicting the three-dimensional structure of a protein from its amino acid sequence alone – is an unsolved problem in computational biology. I will present a novel computational approach to this problem that assembles protein tertiary structure from predicted secondary structure elements. I will demonstrate how this algorithm can be combined with limited EPR- and NMR-spectroscopic

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Lecture on “G PROTEIN-COUPLED RECEPTORS: FROM CLONING TO FUNCTIONAL READ-OUT” at Vanderbilt University

Lecture on “G PROTEIN-COUPLED RECEPTORS: FROM CLONING TO FUNCTIONAL READ-OUT” at Vanderbilt University

Syllabus – CPBP 330 – Special Topics in Protein Biochemistry Dr. Dr. Ines Liebscher Leipzig University, Medical Faculty, Institute of Biochemistry Date 02 – 06 October 2017 Venue Departments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, Room 5131, Nashville, TN 37232-8725 This course will focus on both the theoretical background and practical considerations in modern molecular cloning techniques and the functional read-out of G protein-coupled receptors (GPCR). Upon completion of this course students will be able to successfully apply and optimize cloning techniques in their projects and specifically to plan and evaluate experimental setups to analysis GPCR signal

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International Cooperation: Max-Kade Foundation extends the international fellowship program between Nashville and Leipzig

International Cooperation: Max-Kade Foundation extends the international fellowship program between Nashville and Leipzig

The Center of Structural Biology (Vanderbilt University) and the Institute of Biochemistry (Leipzig University) has secured funds for an international fellowship program from the Max Kade Foundation for the period 2017 to 2021. The Max Kade Foundation takes pride in participating and sponsoring various programs which encourage the exchange of academic ideas among universities and colleges in the United States and in German-speaking countries (www.maxkadefoundation.org). The foundation recently awarded the Vanderbilt-Leipzig research exchange program a second grant to cover the travel and support of six undergraduate student exchanges, as well as two faculty exchanges per year. The students will travel to their respective university and join a research project under a

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Apply for Max Kade Scholarship 2017

Apply for Max Kade Scholarship 2017

Unique Opportunities in Gaining Research Experience in Germany and USA The Max Kade Foundation (MKF, www.maxkadefoundation.org) supports an international research exchange between Vanderbilt and Leipzig Universities. Specifically, MKF provides funds of three undergraduate students from each university to conduct a 10 week summer internship at the respective partner university in 2017 (May – December). The Max Kade Foundation Scholarship Program is open for highly motivated and excellent foreign applicants with a BSc, MSc or equivalent degrees (obtained during the past year). Internship offer in Leipzig for Undergraduate Students from Vanderbilt University  Investigation of allosteric modulation of the human Y4 receptor Prof. Dr. Annette Beck-sickinger / Mario Schubert (Institute of Biophysics) Preparation

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DAAD RISE Programm: CALL 2017

DAAD RISE Programm: CALL 2017

The German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) announced a new call for its program “Research Internships in Science and Engineering” (RISE) for summer internship in 2017. The program works in both ways. German and American Ph.D. students submit internship offers for undergraduates students for a duration of 8 to 12 weeks. German bachelor students can work as research interns at U.S. universities and U.S. undergraduates can work in labs of German universities. The DAAD provides stipends to help cover living expenses, the partner universities and research institutes provide housing assistance. The call is applicable for all fields of biology, chemistry, physics, earth sciences and engineering. Please visit for further

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Short report by Henrike Indrischek: Binding Capability of Visual Arrestins to the Coated Pit Machinery at Vanderbilt University (May 05 – July 28)

Short report by Henrike Indrischek: Binding Capability of Visual Arrestins to the Coated Pit Machinery at Vanderbilt University (May 05 – July 28)

by Henrike Indrischek (phd student at Leipzig University): During my research stay at Vanderbilt university this summer, I worked on the biochemical characterization of the interaction of clathrin and visual arrestin in the laboratory of Seva Gurevich and the modeling of this interaction with computational methods in the laboratory of Jens Meiler. The cytosolic arrestin proteins mediate desensitization of activated G protein-coupled receptors (GPCRs) via competitive binding to the active phosphorylated receptor. As different arrestin conformations can result in specific signaling outcomes, this protein family is an attractive target in drug therapeutics. Non-visual arrestins mediate uptake of the receptors into the cell by also binding clathrin, a component of the endocytosis

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Researchers of Vanderbilt and Leipzig University discover small-molecule modulators of the human Y4 receptor

Researchers of Vanderbilt and Leipzig University discover small-molecule modulators of the human Y4 receptor

(07/04/2016 by Anett Albrecht): A joint publication of Leipzig and Vanderbilt scientists was published in June 2016 in the prestigious journal “PLoS One”. The article continues the series of numerous publications that have emerged already from the long-term collaboration between the Leipzig and Vanderbilt University. Abstract: The human neuropeptide Y4 receptor (Y4R) and its native ligand, pancreatic polypeptide, are critically involved in the regulation of human metabolism by signaling satiety and regulating food intake, as well as increasing energy expenditure. Thus, this receptor represents a putative target for treatment of obesity. With respect to new approaches to treat complex metabolic disorders, especially in multi-receptor systems, small molecule allosteric modulators have been

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Lecture on “Regulation of Exocytosis by inhibitory GPCRs and Gbg subunits” at Leipzig University

Lecture on “Regulation of Exocytosis by inhibitory GPCRs and Gbg subunits” at Leipzig University

Biochemical Colloquium – Special Topics in Biochemistry: Prof. Dr. Heidi Hamm, Vanderbilt University Department of Pharmacology, Nashville, TN, USA Date July 13, 2016 / 3 PM Venue Departments of Bioscience, Pharmacy and Psychology, Institute of Biochemistry, Small Lecture Hall (1st Floor), Brüderstraße 34, 04103 Leipzig, Germany. Backround Prof. Hamms work is focused on understanding the molecular basis of signaling mechanisms mediated by G proteins, which are switch proteins. G proteins are normally inactive, but a receptor that has received a specific signal can activate G proteins, leading to changes in the activity of enzymes that produce second messengers such as cyclic AMP and calcium. The resulting changes in cellular activity underlie

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Course on “In vitro Preparation of Membrane Proteins – from Target DNA to NMR Spectra” at Vanderbilt University

Course on “In vitro Preparation of Membrane Proteins – from Target DNA to NMR Spectra” at Vanderbilt University

Graduate Course – Special Topics in Protein Biochemistry Dr. Peter Schmidt, Leipzig University, Faculty of Medicine, Institute for Medical Physics and Biophysics Date The class will meet on six days in July always 9:35-12:15. Dates will be finalized with class participants. This is a one credit course. Venue Departments of Chemistry, Pharmacology, and Biomedical Informatics, Center for Structural Biology, and Institute of Chemical Biology, 465 21st Ave South, BIOSCI/MRBIII, 5131 MRBIII, Nashville, TN 37232-8725 List of Topics: – Introduction into genetic engineering and recombinant expression (1h) – Membrane proteins, GPCRs, and Y receptors (1h) – Host systems for recombinant membrane protein expression and fermentation process (2h) – Isolation and purification of

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Journal of Biological Chemistry selects Vanderbilt-Leipzig article as paper of the week

Journal of Biological Chemistry selects Vanderbilt-Leipzig article as paper of the week

The joint publication of Hu, Stern, Gimenez, Wanka, Zhu, Rossi, Meister, Inoue, Beck-Sickinger, Gurevich and Wess was named as “Paper of the Week” by the Journal of Biological Chemistry’s editorial board, landing it in the top 2 percent of all papers published over the year in the journal. The joint publication focussed on designer receptors exclusively activated by a designer drug – short DREADDs. This are CNO sensitive designer G protein-coupled receptors which are able to activate heterotrimeric G proteins and also trigger arrestin-dependent signaling. In the present study the development of a mutationally modified version of a non-biased DREADD derived from the M3 muscarinic receptor that can activate Gq/11 with

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Short report by Mario Schubert: HTS to Identify and Characterize Small Molecule Allosteric Modulators of the Human Y4 Receptor at Vanderbilt University (Oct 19 – Dec 15)

Short report by Mario Schubert: HTS to Identify and Characterize Small Molecule Allosteric Modulators of the Human Y4 Receptor at Vanderbilt University (Oct 19 – Dec 15)

During an internship in the lab of Dave Weaver at Vanderbilt University, I got the opportunity to gain more information about the Y4R, by testing a variety of similar structurally related compounds. I was introduced in the workflow of a high throughput screening laboratory. The technical equipment in the Vanderbilt HTS center makes it possible to test thousands of different chemical compounds in a very short time. Therefore, I learned to perform Ca2+-flux based signal transduction screening assays to monitor the activation of the Y4R. Using this system, I could test new hit compounds out of the ongoing screening for activity and selectivity. Furthermore, I tested structurally similar compounds of a

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Short Report by Axel Fischer & Gregory Sliwoski: INTEGRATION OF LIMITED EXPERIMENTAL DATA INTO ENSEMBLE PREDICTION at Leipzig University (Nov 14 – Dec 22)

Short Report by Axel Fischer & Gregory Sliwoski: INTEGRATION OF LIMITED EXPERIMENTAL DATA INTO ENSEMBLE PREDICTION at Leipzig University (Nov 14 – Dec 22)

The objective of our visit was deepening the collaboration between Leipzig University and Vanderbilt University regarding the integration of limited experimental data into protein ensemble prediction. During this visit Axel Fischer presented a method to integrate cross-linking data into protein structure prediction methods at the 5th Symposium on Structural Proteomics in Halle. Following subsequent discussions with Dr. Stefan Kalkhof and Tommy Hofmann, work on an algorithm to efficiently simulate cross-link conformations was continued. In a preliminary study, the algorithm significantly improves the accuracy with which protein structures can be predicted. Additionally, Axel developed an algorithm to integrate solid-state nuclear magnetic resonance (ssNMR) data into the protein structure prediction software BCL::Fold. While

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