Our research focus
In BioMat, we learn from ingenious designs, concepts, and applications found throughout nature to develop unique hybrid living materials (HLMs) using biofilms. These developments will be pursued by combining the following biological, and technical aspects:
- Designing microbial consortia to exploit the metabolic plasticity of photoautotrophic, heterotrophic, and methanotrophic strains for harvesting the maximum amount of CO2 and/or CH4 and solar radiation. Thus, utilizing maximal natural resources and establishing energy-efficient and cost-effective HLMs systems for production purposes.
- Employing microbial biofilms and thereby utilizing the self-immobilization, self-regeneration, and self-adaptation characteristics to retain biomass and generate high-cell-density (HCD) culture. Here, we aim to design structured microbial communities in stable biofilms for efficient light conversion into chemical energy.
- Designing and construction of biofilm-based reactor modules to generate HCD culture for the continuous production of chemicals and energy carriers. At the same time, we will construct prototypes that demonstrate the scaling of HLMs using the numbering-up approach to enhance catalytic surface area and product throughput.
Our Group
GROUP LEADER
Dr. Rohan Karande
Phone: +49 341 97-36593
E-Mail
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Scientific career
Since 2021
Junior Research Group Leader “Bioactive systems for controlled evolutionary materials”, Research and Transfer Center for bioactive Matter (bACTmatter), University of Leipzig, Germany
Since 2021
Guest scientist at the Department of Solar Materials (SoMa),
Helmholtz Centre for Environmental Research, Leipzig, Germany
2014 – 2021
Scientist at the Department of Solar Materials (SoMa), Helmholtz Centre for Environmental Research, Leipzig, Germany
2012 – 2014
Post-doctoral Researcher at the Laboratory of Chemical Biotechnology, TU Dortmund University, Dortmund, Germany
2007 – 2012
Dissertation at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Dortmund, Germany
2004 – 2007
Master of Science in Chemical Engineering (M. Sc.) with specialization in Biochemical Engineering, TU Dortmund University, Dortmund, Germany
1999 – 2003
Bachelor in Chemical Engineering (B.E), Shivaji University (P.V.P Institute of Technology), Kolhapur, India
Selected publications
- Bretschneider L, Heuschkel I, Bühler K, Karande R, Bühler B Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120. Metabolic Engineering, accepted
- Bretschneider L, Heuschkel I, Ahmed A, Bühler K, Karande R, Bühler B
Characterization of different biocatalyst formats for BVMO-catalyzed cyclohexanone oxidation
Biotechnology and Bioengineering - Salamanca D, Bühler K, Engesser K-H, Schmid A, and Karande R*
Whole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes. New Biotechnology, 2021, 60, 200-206. - Heuschkel I, Hanisch S, Volke D, Löfgren E, Hoschek A, Nikel P, Karande R, and Bühler K Continuous production of polycaprolactone monomers from cyclohexanone using engineered Pseudomonas taiwanensis VLB120 biofilms in drip-flow and rotating bed reactors Engineering in Life Sciences, 2021.
- Bretschneider L, Wegner M, Bühler K, Bühler B, and Karande R
One-pot synthesis of 6-aminohexanoic acid from cyclohexane using mixed-species cultures
Microbial Biotechnology, 2020, 1-15 - Heuschkel I, Dagini R, Karande R, and Bühler K The impact of glass material on growth and biocatalytic performance of mixed-species biofilms in capillary reactors for continuous cyclohexanol production Frontiers in Bioengineering and Biotechnology, 2020, 8, 1090-1100
- Hoschek A, Heuschkel I, Schmid A, Bühler B, Karande R, and Bühler K
Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
Bioresource Technology, 2019, 282, 171-178 - Karande R, Salamanca D, Schmid A, and Buehler K Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120 Biotechnology and Bioengineering, 2018, 115, 2, 312-320
- Karande R, Schmid A, and Buehler K
Applications of multiphasic microreactors for biocatalytic reactions
Organic Process Research & Development, 2016, 20, 361-370 - Karande R°, Halan B°, Schmid A, and Buehler K*
Segmented flow is controlling the growth of catalytic biofilms in continuous multiphase microreactors
Biotechnology and Bioengineering, 2014, 111, 1831-1840
°These authors contributed equally to this work - Karande R, Schmid A*, and Buehler K
Miniaturizing Biocatalysis: Enzyme-catalyzed reactions in an aqueous/organic segmented flow capillary microreactor
Advanced Synthesis and Catalysis, 2011, 353, 2511-2521
Patents
- WO/2020/052762
A composition of photoautotrophic microorganisms and chemoheterotrophic microorganisms in a biofilm
Karande R, Hoschek A, Heuschkel I, Bühler K, and Schmid A
2018 - WO/2018/046104
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
Karande R, Salamanca D, Engesser K-H, Buehler K, and Schmid A
2016 - WO/2012/152337
Segmented flow biofilm reactor
Schmid A, Buehler K, and Karande R
2010
PhD STUDENTS
M.Sc. Nina Siebert
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Dipl.-Ing. Alexander Franz
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Dipl.-Ing. Selina Hanisch
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Dipl.-Ing. Valentina Schmitz
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Lea Seibert
Institute of Biochemistry
Johannisallee 21-23
04103 Leipzig
Projects and Cooperations
Projects
REPLACER
The REPLACER project is researching sustainable technologies with which feed proteins or plastic building blocks can be produced from greenhouse gases using algae or bacteria.
The project is funded by M-era.Net and the Free State of Saxony.
Cooperations
Our junior research group works closely with the Working Group Catalytic Biofilms of the Solar Materials Department at the Helmholtz Centre for Environmental Research GmbH (UFZ).