B11 – The role of the ECM during bone regeneration in zebrafish

Adult zebrafish are able to regenerate various organs and tissues after severe trauma. In particular, they are able to rapidly form bone during the process of fin regeneration. We study fin regeneration in order to better understand the role of the ECM during bone formation. In this context, we aim at improving bone formation with the help of artificial ECM.


  1. Dietrich K, Fiedler IA, Kurzyukova A, López-Delgado AC, McGowan LM, Geurtzen K, Hammond CL, Busse B, Knopf F. Skeletal biology and disease modeling in zebrafish. J Bone Miner Res 2021. doi: 10.1002/jbmr.4256 
  2. Schmidt JR*, Geurtzen K*, von Bergen M, Schubert K# and Knopf F#. Glucocorticoid treatment leads to aberrant ion and macromolecular transport in regenerating zebrafish fins. Front Endocrinol. 2019; 10:674. * equal contribution # co-corresponding authors.
  3. Geurtzen K, Knopf F. Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue. J Vis Exp. 2018; 140:e58429.
  4. Geurtzen K, Vernet A, Freidin A, Rauner M, Hofbauer LC, Schneider JE, Brand M, Knopf F. Immune Suppressive and Bone Inhibitory Effects of Prednisolone in Growing and Regenerating Zebrafish Tissues. J Bone Miner Res 2017, 32: 2476-2488.
  5. Geurtzen K*, Knopf F*, Wehner D, Huitema LF, Schulte-Merker S, Weidinger G. Mature steoblasts dedifferentiate in response to traumatic bone injury in the zebrafish fin and kull. Development. 2014;141:2225-34. (*geteilte Erstautorschaft).
  6. Gupta M, Brand M. Identification and Expression Analysis of Zebrafish Glypicans during Embryonic Development. PLoS One. 2013;8:e80824.
    Boekel C, Brand M. Generation and interpretation of FGF morphogen gradients in vertebrates. Curr Opin Gen Dev. 2013;23:415-22.
  7. Knopf F, Hammond C, Chekuru A, Kurth T, Hans S, Weber CW, Mahatma G, Fisher S, Brand M, Schulte-Merker S, Weidinger G. Bone regenerates via dedifferentiation of osteoblastsen the zebrafish fin. Dev Cell. 2011;20:713-24.
  8. Nowak M, Yu SR, Gupta M, Machate A, Brand M. Interpretation of the FGF8 morphogen gradient is regulated by endocytic trafficking. Nat Cell Biol. 2011;13:153-8.
  9. Yu SR, Burkhardt M, Nowak M, Ries J, Petrášek Z, Scholpp S, Schwille P*, Brand M.* FGF8 morphogen gradient is formed by a source-sink mechanism with freely-diffusing molecules. Nature. 2009;461:533-6. (*geteilte Seniorautorschaft).
  10. Ries J, Yu SR, Burkhardt M, Brand M*, Schwille P*. Modular scanning FCS quantifies ligand-receptor interactions in live multicellular organisms. Nat Methods. 2009;6:643-5. (*geteilte Seniorautorschaft).
  11. Raible F, Brand M. Divide et Impera-the midbrain-hindbrain boundary and its organizer. Trends Neurosci. 2004;27:727-34.
    Scholpp S, Brand M. Endocytosis controls spreading and effective signaling range of FGF8. Curr Biol. 2004;14:1834-41.


Prof. Dr. Michael Brand

Technische Universität Dresden
CRTD – Zentrum für Regenerative Therapien TU Dresden
Fetscherstraße 105, 01307 Dresden

Phone: +49 (0)351 458 82300
E-Mail: michael.brand@tu-dresden.de
Web: crt-dresden.de/research/research-groups

Jun.-Prof. Dr. Franziska Knopf

Technische Universität Dresden
CRTD – Zentrum für Regenerative Therapien TU Dresden
Fetscherstraße 105, 01307 Dresden

Phone: +49 (0)351 458 82336
E-Mail: franziska.knopf@tu-dresden.de
Web: crt-dresden.de/research/research-groups